Description

4-Methylumbelliferone-3-Acetic Acid n-Succinimidyl Ester is a high-purity, activated ester derivative of 4-methylumbelliferone (4-MU) designed for efficient bioconjugation. This compound is a critical reagent for researchers and manufacturers developing fluorescent probes, enzyme substrates, and diagnostic assays. It is primarily used by professionals in the life sciences, pharmaceutical R&D, and diagnostic kit production sectors who require reliable, high-quality labeling reagents for proteins, antibodies, and other biomolecules.

Application

• Fluorescent Probe Synthesis: Acts as a key intermediate for creating sensitive fluorescent labels and tags for biomolecules.
• Enzyme Substrate Development: Used to synthesize fluorogenic substrates for detecting enzymatic activity (e.g., proteases, esterases) in high-throughput screening and diagnostic assays.
• Bioconjugation & Labeling: The activated NHS ester group enables straightforward, amine-reactive coupling to proteins, peptides, and antibodies for fluorescence-based detection.
• Diagnostic Assay Manufacturing: A vital component in the production of research-use-only (RUO) and in-vitro diagnostic (IVD) kits for clinical and research laboratories.
• Biochemical Research: Employed in cell biology and molecular biology studies for tracking and visualization applications.
• Chemical Biology Tool Development: Serves as a building block for designing activity-based probes and other chemical tools to study biological processes.

Basic Information

Product Name	4-Methylumbelliferone-3-Acetic Acid n-Succinimidyl Ester
CAS No.	96735-88-5
Molecular Formula	C16H13NO7
Molecular Weight	331.28 g/mol
Synonyms	4-MU-3-Acetic Acid NHS Ester; 4-Methylumbelliferyl-3-acetic Acid N-Hydroxysuccinimide Ester; 7-Hydroxy-4-methyl-2-oxo-2H-chromen-3-ylacetic Acid 2,5-Dioxopyrrolidin-1-yl Ester; 3-Carboxymethyl-4-methylumbelliferone N-Succinimidyl Ester; 4-Methylumbelliferone-3-acetic Acid N-Succinimidyl Ester; 4-MU-3-Ac-OSu; 4-MU-3-Ac-NHS
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferone-3-Acetic Acid n-Succinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and reactivity. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.