Description

Hippuryl-Arg-Gly CAS NO 96723-71-6 is a synthetic tripeptide derivative widely recognized as a specific chromogenic substrate for the enzyme thrombin. Its primary value lies in enabling precise, colorimetric measurement of thrombin activity, which is critical for research and diagnostic applications in hemostasis. This compound is essential for pharmaceutical R&D laboratories, clinical diagnostics manufacturers, and academic institutions focused on cardiovascular disease, coagulation disorders, and protease inhibitor screening.

Application

• Thrombin Activity Assays: Primary chromogenic substrate for the quantitative determination of thrombin concentration and enzymatic activity in biochemical research.
• Diagnostic Kit Manufacturing: Key raw material in the production of in-vitro diagnostic (IVD) kits for monitoring blood coagulation parameters, such as prothrombin time (PT) and activated partial thromboplastin time (aPTT).
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate novel direct thrombin inhibitors (DTIs) and anticoagulant drug candidates.
• Clinical Research: Employed in studies investigating thrombotic and bleeding disorders, including deep vein thrombosis (DVT), pulmonary embolism, and hemophilia.
• Quality Control in Biologics: Applied in the QC testing of heparin products and other anticoagulants to measure residual thrombin-like activity.
• Enzyme Kinetic Studies: Serves as a specific substrate for detailed kinetic analysis of thrombin's catalytic mechanism and inhibition constants (Ki).

Basic Information

Product Name	Hippuryl-Arg-Gly
CAS No.	96723-71-6
Molecular Formula	C₂₀H₂₉N₇O₆
Molecular Weight	463.49 g/mol
Synonyms	Hippuryl-L-arginyl-glycine; Hippuryl-Arg-Gly-OH; H-Arg-Gly-OH·Hip; Bz-Gly-Arg-Gly; Thrombin Chromogenic Substrate S-2238 Analog; N-Benzoylglycyl-L-arginylglycine; Chromogenic Substrate for Thrombin; Bz-Gly-Arg-Gly-OH
EINECS	Contact for details

Quality Control

Our Hippuryl-Arg-Gly is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific activity testing in thrombin assays. We provide full traceability and a detailed Certificate of Analysis (COA) with each shipment, ensuring compliance with the stringent requirements of pharmaceutical and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Optical Rotation	-25° to -30° (c=1 in H₂O)
Assay (Enzymatic)	≥ 95% of theoretical activity
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.