Description

H-Ala-Amc Tfa is a specialized, high-purity fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like aminopeptidases. It serves as an essential tool in biochemical research, pharmaceutical development, and diagnostic applications where accurate, real-time monitoring of enzymatic reactions is paramount.

Application

• Biochemical Research: As a fluorogenic substrate for studying the kinetics and inhibition of aminopeptidases and other proteolytic enzymes.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Development: Employed in the development of clinical diagnostic kits for detecting specific protease activities associated with diseases.
• Enzyme Characterization: For determining the specificity and catalytic efficiency of purified proteases in academic and industrial R&D settings.
• Quality Control: Utilized in biopharmaceutical manufacturing to monitor and validate the activity of enzyme-based products or processes.

Basic Information

Product Name	H-Ala-Amc Tfa
CAS No.	96594-10-4
Molecular Formula	C16H18F3N3O5
Molecular Weight	389.33 g/mol
Synonyms	L-Alanine 7-amido-4-methylcoumarin Trifluoroacetate; Ala-AMC TFA; H-Ala-AMC.TFA; Alanine-7-amido-4-methylcoumarin TFA Salt; 7-(L-Alanylamino)-4-methylcoumarin trifluoroacetate; Aminopeptidase Substrate Ala-AMC; Fluorogenic Substrate for Aminopeptidase; N-(7-Amino-4-methylcoumarin)-L-alanine trifluoroacetate
EINECS	Contact for details

Quality Control

Our H-Ala-Amc Tfa is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to rigorous internal standards suitable for research and development use, with specifications available to support your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.