Description

α-1,3-Galactosyl Transferase Recomb. From E. Coli is a highly purified recombinant enzyme used for the specific transfer of galactose residues in an α-1,3 linkage. This enzyme is critical for the synthesis and study of glycoconjugates, particularly in xenotransplantation research and the development of biotherapeutics. It is an essential tool for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors requiring precise enzymatic glycosylation.

Application

• Glycobiology Research: Fundamental tool for synthesizing and analyzing α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) in vitro.
• Xenotransplantation Studies: Used to create and characterize glycans relevant to hyperacute rejection mechanisms.
• Diagnostic Reagent Production: Key component in manufacturing enzymatic assay kits for detecting anti-α-gal antibodies.
• Biotherapeutics Development: Employed in the enzymatic remodeling of therapeutic glycoproteins to modify immunogenicity or pharmacokinetic properties.
• Carbohydrate Synthesis: Enables the chemoenzymatic synthesis of complex oligosaccharides and neoglycoconjugates for vaccine and drug discovery.
• Quality Control: Acts as a reference standard in QC labs for assays related to glycosyltransferase activity.

Basic Information

Product Name	α-1,3-Galactosyl Transferase Recomb. From E. Coli
CAS No.	96477-57-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	α-1,3-Galactosyltransferase; α1,3GalT; α1,3GT; GGTA1; Recombinant α-Galactosyltransferase; EC 2.4.1.151; UDP-galactose:β-D-galactosyl-1,4-N-acetyl-D-glucosaminide α-1,3-galactosyltransferase; α-Gal Transferase; Xenotransplantation Antigen Synthesizing Enzyme
EINECS	Contact for details

Quality Control

Our recombinant enzyme is produced under stringent conditions to ensure high purity, specific activity, and lot-to-lot consistency. Quality is verified through a comprehensive battery of tests including SDS-PAGE, activity assays, and endotoxin level checks. A Certificate of Analysis (COA) detailing purity, activity, and storage buffer composition is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Aliquot upon receipt for optimal performance.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Purity (SDS-PAGE)	≥ 90%
Specific Activity	≥ XX U/mg (Contact for details)
Protein Concentration	XX mg/mL (Contact for details)
Buffer	XX mM Tris-HCl, XX mM NaCl, XX% Glycerol, pH X.X
Endotoxin Level	< 1.0 EU/µg
Identification (Activity)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.