Description

Photobiotin Acetate is a chemically modified biotin derivative designed for photoactivatable cross-linking applications. This compound is essential for researchers and developers requiring precise, light-induced labeling of biomolecules, offering a critical tool for advanced biochemical analysis and diagnostics. It is primarily utilized in the life sciences sector for applications such as nucleic acid and protein labeling, affinity chromatography, and the development of sensitive detection assays.

Application

• Nucleic Acid Labeling & Detection: Used as a photoactivatable probe for non-radioactive labeling of DNA and RNA for blotting, in situ hybridization, and microarray analysis.
• Protein Biotinylation & Pull-Down Assays: Enables site-specific biotin tagging of proteins via light activation for subsequent purification or detection using streptavidin conjugates.
• Affinity Chromatography Probe Synthesis: Serves as a key reagent for preparing photo-crosslinkable biotinylated ligands immobilized on avidin/streptavidin resins.
• Diagnostic Kit Development: Employed in the manufacturing of sensitive immunoassays and biosensors that require stable, high-affinity biotin-streptavidin interactions.
• Cell Surface Labeling: Facilitates the study of membrane proteins and receptors through light-controlled biotinylation of live cells.
• Molecular Biology Research: A vital tool for probing biomolecular interactions, including protein-protein and protein-nucleic acid complexes.

Basic Information

Product Name	Photobiotin Acetate
CAS No.	96087-38-6
Molecular Formula	C26H39N7O6S
Molecular Weight	577.70 g/mol
Synonyms	N-[2-[2-[2-[5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethyl]-4-azido-2-hydroxybenzamide Acetate; N-(4-Azido-2-hydroxybenzoyl)-N'-biotinyl-1,8-diamino-3,6-dioxaoctane Acetate; Biotinylated Photoactivatable Reagent; Photoprobe Biotin Acetate; Photoactivatable Biotin Acetate; Biotin-XX-SSE Acetate (photoactivatable analog); Biotin Hydrazide Photoactivatable Derivative
EINECS	Contact for details

Quality Control

Our Photobiotin Acetate is manufactured under strict quality controls to ensure high purity and batch-to-batch consistency suitable for research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard labeling assays. We adhere to ISO 9001 quality management standards, and our production environment is designed to meet the needs of sensitive biochemical reagent manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated at -20°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Photoactivation Efficiency	Functional in standard labeling assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.