Description

n-(4-Azido-2-Nitrophenyl)-N'-(3-Biotinylamino-Propyl)-N'-Methyl-1,3-Propanediamine Acetate Salt is a specialized heterobifunctional crosslinking reagent that combines a photoactivatable aryl azide group with a biotin moiety. This compound is critical for photoaffinity labeling and the selective capture and detection of biomolecular interactions. It is primarily used by researchers and manufacturers in biochemistry, proteomics, and molecular biology for applications such as protein-protein interaction studies and affinity purification.

Application

• Photoaffinity Labeling: Used to covalently tag and study protein-ligand or protein-protein interactions upon UV light activation.
• Affinity Purification & Pull-Down Assays: The biotin tag enables efficient capture and isolation of target complexes using streptavidin-coated beads or columns.
• Proteomics Research: A key tool for identifying and mapping transient or weak biomolecular interactions in complex biological samples.
• Bioconjugation & Probe Development: Serves as a building block for creating advanced diagnostic probes and biosensors.
• Structural Biology Studies: Assists in elucidating binding sites and interaction interfaces of macromolecules.
• Drug Discovery Platforms: Employed in target identification and validation stages to understand drug mechanism of action.

Basic Information

Item	Details
Product Name	n-(4-Azido-2-Nitrophenyl)-N'-(3-Biotinylamino-Propyl)-N'-Methyl-1,3-Propanediamine Acetate Salt
CAS No.	96087-37-5
Molecular Formula	C26H40N10O5S • C2H4O2
Molecular Weight	Contact for details
Synonyms	ANB-NOS-Biotin; 4-Azido-2-nitrophenyl biocytin; Biotin-ANB-NOS; N-[4-(Azido-2-nitrophenyl)]-N-biotinyl-1,3-propanediamine acetate salt; Biotinylated aryl azide; Heterobifunctional crosslinker ANB-NOS-Biotin; Photoactivatable biotin reagent; Biotin-XX-ANB-NOS
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical reagent adheres to stringent quality protocols. Every batch is characterized and tested to ensure consistent performance and lot-to-lot reproducibility for critical research applications. A comprehensive Certificate of Analysis (COA) detailing purity, functional validation, and spectral data (NMR, MS) is provided with each shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Validated by streptavidin binding assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.