Description

H-Hexahydrotyrosyl-Alanyl-Arginine-4-Nitroanilide is a specialized synthetic peptide derivative designed for high-precision biochemical research and pharmaceutical development. This compound serves as a critical chromogenic substrate for the study and quantification of specific protease enzyme activity, particularly thrombin and related serine proteases. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors requiring reliable and sensitive assay components.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of thrombin and trypsin-like enzyme activity in research and diagnostic kits.
• Pharmaceutical R&D: Used in high-throughput screening (HTS) and kinetic studies to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Manufacturing: A key raw material in the production of clinical chemistry reagents for coagulation testing and other enzymatic diagnostic panels.
• Biochemical Research: Tool for studying enzyme kinetics, specificity, and mechanism of action in academic and industrial laboratories.
• Quality Control: Employed in the QC testing of biopharmaceuticals, such as thrombin products, to ensure batch-to-batch consistency and potency.

Basic Information

Product Name	H-Hexahydrotyrosyl-Alanyl-Arginine-4-Nitroanilide
CAS No.	95752-07-1
Molecular Formula	C26H41N9O7
Molecular Weight	567.66 g/mol
Synonyms	H-HHT-A-R-pNA; Hexahydro-Tyr-Ala-Arg-pNA; Thrombin Substrate I (Chromogenic); Tosyl-Gly-Pro-Arg-p-nitroanilide acetate salt (related); Bz-Phe-Val-Arg-pNA (similar class); S-2238 (alternative chromogenic substrate); Chromogenic Peptide Substrate for Thrombin; p-Nitroanilide Peptide Derivative
EINECS	Contact for details

Quality Control

Our H-Hexahydrotyrosyl-Alanyl-Arginine-4-Nitroanilide is manufactured under strict quality management systems to ensure the high purity and consistency required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay systems. We adhere to relevant guidelines for research-grade biochemicals to guarantee reliable and reproducible results in your laboratory.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); allow the sealed container to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Assay (Enzymatic)	≥ 90% of theoretical activity
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.