Description

Suc-Val-Pro-Phe-Pna is a synthetic peptide derivative, specifically a chromogenic substrate designed for enzymatic assays. This compound is a critical reagent for researchers and quality control laboratories requiring precise measurement of protease activity. Its primary value lies in enabling sensitive and specific detection of enzymes like chymotrypsin and related proteases through a colorimetric readout. Industries that rely on this product include pharmaceutical R&D, biotechnology for enzyme characterization, and diagnostic kit manufacturing.

Application

• Enzymatic Assay Substrate: Primary use as a chromogenic substrate for the spectrophotometric determination of chymotrypsin and chymotrypsin-like protease activity.
• Biochemical Research: A key tool for studying protease kinetics, inhibition, and enzyme mechanism in academic and industrial research settings.
• Pharmaceutical Quality Control: Used in potency testing and stability studies of enzyme-based pharmaceutical products.
• Diagnostic Development: Serves as a core component in the development of clinical diagnostic assays for detecting specific protease levels.
• Protease Inhibitor Screening: Facilitates high-throughput screening (HTS) campaigns to identify and characterize novel protease inhibitors for drug discovery.
• Food Science & Biotechnology: Potential application in monitoring proteolytic activity during fermentation or food processing.

Basic Information

Product Name	Suc-Val-Pro-Phe-Pna
CAS No.	95192-11-3
Molecular Formula	C30H38N6O7
Molecular Weight	594.66 g/mol
Synonyms	Suc-Val-Pro-Phe-pNA; N-Succinyl-Val-Pro-Phe-p-nitroanilide; Suc-VPF-pNA; N-Succinyl-Valyl-Prolyl-Phenylalanyl-p-Nitroanilide; Chymotrypsin Substrate Suc-VPF-pNA; Protease Substrate 95192-11-3; Suc-Val-Pro-Phe-p-Nitroanilide; Chromogenic Substrate for Chymotrypsin
EINECS	Contact for details

Quality Control

Our Suc-Val-Pro-Phe-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and assay results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Assay (Enzymatic)	≥ 95% of theoretical activity
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.