Description

Restriction Endonuclease Nci I From Neis is a highly specific enzyme used for precise DNA cleavage at defined recognition sequences. This product is critical for molecular biology research, genetic engineering, and biotechnological applications requiring accurate DNA manipulation. It is essential for laboratories and industrial facilities involved in recombinant DNA technology, genome mapping, and synthetic biology.

Application

• Molecular Cloning: Essential for the precise cutting of DNA fragments for insertion into vectors.
• Genetic Engineering: Used in the construction of recombinant DNA molecules for research and bioproduction.
• Genome Analysis: A key tool for restriction fragment length polymorphism (RFLP) analysis and genome mapping.
• Diagnostic Assay Development: Employed in the creation of molecular diagnostic tests that rely on specific DNA sequence detection.
• Synthetic Biology: Facilitates the assembly of genetic circuits and synthetic pathways in engineered organisms.
• Quality Control in Biomanufacturing: Used to verify plasmid identity and integrity in upstream bioprocessing.

Basic Information

Product Name	Restriction Endonuclease Nci I From Neis
CAS No.	95076-97-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Nci I Restriction Enzyme; Endodeoxyribonuclease Nci I; NciI; Nci I (Neisseria cinerea); Restriction Enzyme Nci I; Type II Restriction Enzyme Nci I; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Nci I is produced and tested under stringent conditions to ensure high activity, specificity, and purity, free from contaminating nucleases. Each batch is validated for its precise cleavage pattern and absence of non-specific activity. Certificates of Analysis (COA) detailing activity units, purity, and stability are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The supplied storage buffer is optimized to maintain enzyme activity.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves DNA specifically at the recognized sequence
Specific Activity	≥ 5,000 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Contaminating Nuclease Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.