Description

C6-Nbd-Ceramide CAS NO 94885-02-6 is a fluorescently labeled lipid derivative, specifically a ceramide analog tagged with the NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) fluorophore on a C6 acyl chain. This compound is a critical tool for real-time visualization and tracking of lipid metabolism, trafficking, and membrane dynamics within live cells. It is essential for researchers and developers in the life sciences, particularly in cell biology, lipidomics, and pharmaceutical development, where understanding intracellular lipid pathways is paramount.

Application

• Live-Cell Imaging of Lipid Trafficking: Used as a vital fluorescent probe to trace the movement and localization of ceramide and sphingolipids in the Golgi apparatus, endoplasmic reticulum, and plasma membrane in real time.
• Sphingolipid Metabolism Studies: Enables the investigation of ceramide-related metabolic pathways, including synthesis, degradation, and conversion to other sphingolipids like sphingomyelin and glycosphingolipids.
• Membrane Biophysics Research: Serves as a tool to study lipid bilayer properties, membrane fluidity, and the formation of lipid rafts and microdomains.
• Drug Discovery & Toxicology Screening: Applied in high-content screening assays to evaluate the effects of pharmaceutical compounds or toxins on lipid homeostasis and organelle function.
• Apoptosis (Programmed Cell Death) Research: Utilized to monitor the role of ceramide, a key signaling molecule, in initiating and regulating apoptotic pathways.
• Fluorescence Microscopy & Flow Cytometry: Acts as a standard fluorescent marker for quantitative analysis of lipid content and distribution using advanced imaging and cytometry techniques.

Basic Information

Item	Details
Product Name	C6-Nbd-Ceramide
CAS No.	94885-02-6
Molecular Formula	C34H55N3O6
Molecular Weight	601.83 g/mol
Synonyms	N-[6-[(7-Nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphingosine; C6-NBD-Ceramide; NBD C6-Ceramide; 6-((N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosine; NBD-Ceramide (C6); Fluorescent Ceramide C6; D-erythro-C6-NBD-Ceramide; Ceramide analog NBD C6
EINECS	Contact for details

Quality Control

Our C6-Nbd-Ceramide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, mass spectrometry (MS) for identity confirmation, and fluorescence spectroscopy to ensure optimal performance in imaging applications. Certificates of Analysis (COA) with detailed chromatograms and spectral data are available upon request to support your research and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. For long-term storage, solutions should be prepared in anhydrous DMSO or ethanol and kept at -80°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Excitation/Emission Maxima	~466 nm / ~536 nm (in methanol)
Solubility	Soluble in DMSO, DMF, ethanol, chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.