Description

Gtp-γ-S Tetralithium Salt is a high-purity biochemical reagent, specifically the tetralithium salt of guanosine-5'-triphosphate, γ-thio analog. This compound serves as a crucial, non-hydrolyzable analog of GTP, making it an essential tool for studying G-protein coupled receptor (GPCR) signaling pathways and other GTPase-dependent processes. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic manufacturers engaged in molecular biology, enzymology, and signal transduction research.

Application

• Biochemical Research: As a stable GTP analog to probe the mechanism of G-proteins, GTPases, and other nucleotide-binding proteins.
• Enzyme Assays: Used in kinetic studies and activity assays for enzymes that utilize GTP, such as kinases and synthetases.
• Signal Transduction Studies: Essential for investigating GPCR pathways and intracellular signaling cascades without signal termination via hydrolysis.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify modulators of GTP-binding protein activity.
• Diagnostic Development: Can be used in the development of assays for diseases linked to GTPase dysfunction.
• Structural Biology: Aids in X-ray crystallography and NMR studies to determine protein structures in their GTP-bound state.

Basic Information

Product Name	Gtp-γ-S Tetralithium Salt
CAS No.	94825-44-2
Molecular Formula	C10H12Li4N5O13P3S
Molecular Weight	599.04 g/mol (anhydrous basis)
Synonyms	Guanosine 5'-[γ-thio]triphosphate tetralithium salt; GTPγS tetralithium salt; Guanosine-5'-(3-thiotriphosphate) tetralithium salt; 5'-Guanosylphosphorothioate tetralithium salt; Gtp-γ-S Li4 salt; Non-hydrolyzable GTP analog; G-protein activator; EC 3.6.5
EINECS	Contact for details

Quality Control

Our Gtp-γ-S Tetralithium Salt is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high biochemical activity. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and biological activity is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0 %
Biological Activity	Conforms (by functional assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.