Description

Phenyl-D5-7-Hydroxywarfarin is a stable isotope-labeled analog of 7-hydroxywarfarin, where five hydrogen atoms on the phenyl ring are replaced with deuterium (D). This compound is essential as an internal standard in quantitative bioanalytical methods, providing unmatched accuracy and reliability for pharmacokinetic and metabolic studies. It is primarily used by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities specializing in LC-MS/MS and GC-MS applications.

Application

• Internal Standard for Mass Spectrometry: Critical for the precise quantification of 7-hydroxywarfarin and related metabolites in complex biological matrices (e.g., plasma, urine) using LC-MS/MS.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Enables accurate tracking of warfarin metabolism pathways, supporting ADME (Absorption, Distribution, Metabolism, Excretion) research.
• Clinical Research and Therapeutic Drug Monitoring (TDM): Used in assays to monitor patient plasma levels of warfarin metabolites for personalized anticoagulant therapy.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of process-related impurities and degradants in warfarin API manufacturing.
• Forensic and Toxicological Analysis: Supports investigations into drug-related incidents by providing a definitive reference standard for warfarin metabolite confirmation.
• Biomarker Research: Facilitates studies on the correlation between warfarin metabolism genotypes (e.g., CYP2C9) and phenotypic metabolic outcomes.

Basic Information

Item	Detail
Product Name	Phenyl-D5-7-Hydroxywarfarin
CAS No.	94820-65-2
Molecular Formula	C19H11D5O5
Molecular Weight	331.35 g/mol (for C19H11D5O5)
Synonyms	7-Hydroxywarfarin-d5; 4-Hydroxy-3-(3-oxo-1-phenylbutyl)-2H-1-benzopyran-2-one (phenyl-d5); Warfarin 7-hydroxy metabolite-d5; 3-(α-Acetonylbenzyl-d5)-4-hydroxycoumarin; 7-OH-Warfarin-d5; (RS)-7-Hydroxywarfarin-d5; 7-Hydroxy-3-(α-phenyl-d5-β-acetylethyl)-4-hydroxycoumarin
EINECS	Contact for details

Quality Control

Every batch of Phenyl-D5-7-Hydroxywarfarin is manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Our products undergo rigorous testing, including NMR, HPLC, and MS analysis, to confirm identity, isotopic enrichment (typically ≥98 atom % D), and chemical purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain integrity. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity	≥98 atom % D
Chemical Purity	≥95%
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.