Description

Dutp, 100Mm Solution is a ready-to-use, standardized aqueous solution of 2'-Deoxyuridine 5'-Triphosphate, a critical nucleotide essential for DNA synthesis and repair processes. This product is formulated for high-throughput molecular biology applications where precise concentration and batch-to-batch consistency are paramount for reliable experimental results. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in PCR, sequencing, and various enzymatic assays.

Application

• Polymerase Chain Reaction (PCR): Used as a substrate for DNA polymerases in amplification protocols, including qPCR and RT-PCR.
• DNA Sequencing: Serves as a standard nucleotide in Sanger sequencing and next-generation sequencing (NGS) workflows.
• Enzymatic DNA Synthesis: Acts as a building block in in vitro enzymatic DNA synthesis and cDNA library construction.
• Mutagenesis Studies: Employed in site-directed mutagenesis and other techniques requiring controlled nucleotide incorporation.
• Diagnostic Assay Development: A key component in the formulation of master mixes for clinical and veterinary diagnostic tests.
• Biochemical Research: Used to study enzyme kinetics, substrate specificity of polymerases, and nucleic acid metabolism.

Basic Information

Product Name	Dutp, 100Mm Solution
CAS No.	94736-09-1
Molecular Formula	C9H15N2O14P3
Molecular Weight	468.14 g/mol (Free Acid)
Synonyms	2'-Deoxyuridine-5'-triphosphate; dUTP; Deoxyuridine triphosphate; 2'-Deoxyuridine 5'-(tetrahydrogen triphosphate); dUTP, sodium salt; dUTP solution; Deoxyuridylate triphosphate; 5'-dUTP
EINECS	Contact for details

Quality Control

Our Dutp, 100Mm Solution is manufactured under stringent quality systems. Each batch is tested to ensure it meets high-purity standards suitable for sensitive molecular biology applications. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as concentration, pH, purity by HPLC, and absence of contaminating nucleases. We support compliance with relevant ISO and GMP guidelines for raw materials used in diagnostic and research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The solution is stable under these conditions for up to 24 months from the date of manufacture.

Specification

Item	Specification
Appearance	Clear, colorless solution
Concentration	100 mM ± 5%
pH	7.0 - 7.5
Purity (HPLC)	≥ 98%
Identification (IR/UV)	Conforms
Endonuclease Activity	Not detected
Exonuclease Activity	Not detected
Filter Sterilization	0.2 µm filtered

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.