Description

Phenolphthalein Diphosphate Calcium Salt is a high-purity biochemical reagent and a key derivative of phenolphthalein. This compound is valued for its role as a chromogenic substrate in enzymatic assays, particularly for the detection of alkaline phosphatase activity. It is essential for researchers and manufacturers in the life sciences, diagnostic, and pharmaceutical industries requiring precise and reliable analytical substrates.

Application

• Diagnostic Reagent: Primary use as a chromogenic substrate in clinical chemistry for detecting alkaline phosphatase (ALP) in ELISA, immunoblotting, and other enzymatic assays.
• Biochemical Research: Used in laboratory research to study enzyme kinetics, inhibition, and cellular signaling pathways involving phosphatases.
• Histochemistry & Staining: Employed in histological staining techniques to localize and visualize alkaline phosphatase activity in tissue samples.
• Pharmaceutical Analysis: Acts as a critical component in quality control testing for drug development and manufacturing processes.
• Food Safety Testing: Utilized in enzymatic methods for detecting microbial contamination or specific enzymatic activities in food products.
• Water Quality Monitoring: Applied in environmental testing kits to assess microbial or enzymatic activity as an indicator of contamination.

Basic Information

Product Name	Phenolphthalein Diphosphate Calcium Salt
CAS No.	94465-66-4
Molecular Formula	C20H14CaO10P2 • xH2O
Molecular Weight	Anhydrous: 516.33 g/mol
Synonyms	Phenolphthalein Diphosphate Ca Salt; Phenolphthalein Bisphosphate Calcium Salt; Calcium Phenolphthalein Diphosphate; ALP Substrate; Phosphatase Substrate; Phenolphthalein Phosphate Calcium; 3,3-Bis(4-hydroxy-3-phosphonatophenyl)-1(3H)-isobenzofuranone Calcium Salt; NSC 113939
EINECS	Contact for details

Quality Control

Our Phenolphthalein Diphosphate Calcium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and performance. Ensure the container is sealed immediately after use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, slightly soluble in ethanol
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.5%
Enzymatic Activity	Suitable for use as ALP substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.