Description

6-Hexadecanoylamido-4-Methylumbelliferyl-β-D-Galactopyranoside is a specialized fluorogenic substrate designed for the sensitive detection of β-galactosidase activity. This compound matters for researchers and quality control professionals who require precise, high-sensitivity enzymatic assays. It is primarily needed in the biotechnology, pharmaceutical, and diagnostic industries for applications such as gene expression studies, cell-based reporter assays, and enzyme-linked immunosorbent assays (ELISA).

Application

• Biochemical Research: A key fluorogenic substrate for detecting and quantifying β-galactosidase activity in vitro.
• Molecular & Cell Biology: Used in reporter gene assays (e.g., lacZ gene) to monitor gene expression and promoter activity in bacterial and mammalian cells.
• Diagnostic Development: Employed in the development of sensitive ELISA and other enzymatic immunoassays for clinical diagnostics.
• High-Throughput Screening (HTS): Suitable for automated screening platforms in drug discovery to identify modulators of β-galactosidase or related pathways.
• Microbiology: Used as a differential substrate in microbial identification and studies of bacterial metabolism.
• Enzyme Kinetics & Characterization: Essential for studying the kinetics, inhibition, and specificity of β-galactosidase enzymes.

Basic Information

Product Name	6-Hexadecanoylamido-4-Methylumbelliferyl-β-D-Galactopyranoside
CAS No.	94452-17-2
Molecular Formula	C32H47NO10
Molecular Weight	605.72 g/mol
Synonyms	6-Palmitoylamido-4-methylumbelliferyl-β-D-galactopyranoside; Pal-AMUG; 6-Hexadecanoylamido-4-methylumbelliferyl-β-D-galactoside; 4-Methylumbelliferyl-6-palmitamido-β-D-galactopyranoside; β-Galactosidase Fluorogenic Substrate (Palmitoylated); 6-O-Palmitoyl-4-methylumbelliferyl-β-D-galactopyranoside; AMUG-Palmitate; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our 6-Hexadecanoylamido-4-Methylumbelliferyl-β-D-Galactopyranoside is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high-purity standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Fluorogenic Activity	Conforms to functional test
Solubility	Soluble in DMSO, DMF; slightly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.