Description

L-Methionine 4-Methyl-7-Coumarinylamide Trifluoroacetate is a specialized fluorogenic substrate and biochemical reagent of high purity. This compound is critical for enzymatic activity assays, particularly for proteases like methionine aminopeptidase, where it serves as a sensitive and selective substrate. It is primarily utilized by research institutions, pharmaceutical R&D laboratories, and diagnostic kit manufacturers engaged in enzyme kinetics, high-throughput screening, and biochemical pathway analysis.

Application

• Protease Activity Assays: A key fluorogenic substrate for monitoring the activity of methionine aminopeptidases and related enzymes in real-time.
• Biochemical Research: Used in fundamental studies of enzyme mechanisms, inhibition, and kinetics within academic and industrial research settings.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic enzyme inhibitors.
• Diagnostic Development: Serves as a critical component in developing sensitive diagnostic assays for enzymatic biomarkers.
• Cell Biology Studies: Applied in cellular assays to investigate methionine aminopeptidase function and its role in post-translational modifications.

Basic Information

Product Name	L-Methionine 4-Methyl-7-Coumarinylamide Trifluoroacetate
CAS No.	94367-35-8
Molecular Formula	C₁₆H₁₉F₃N₂O₅
Molecular Weight	376.33 g/mol
Synonyms	L-Methionine 7-amido-4-methylcoumarin trifluoroacetate; Methionine-AMC TFA; Met-AMC TFA; L-Met-AMC trifluoroacetate; 4-Methyl-7-coumarylamide-L-methionine TFA salt; Fluorogenic methionine substrate; Methionine aminopeptidase substrate; MAP substrate
EINECS	Contact for details

Quality Control

Our L-Methionine 4-Methyl-7-Coumarinylamide Trifluoroacetate is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to guarantee compliance with your research-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Fluorogenic Activity	Functional in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.