Description

Suc-Leu-Tyr-Amc CAS NO 94367-20-1 is a fluorogenic peptide substrate specifically designed for the sensitive detection and quantification of protease activity, particularly chymotrypsin-like enzymes. This compound is critical for biochemical research and pharmaceutical development, enabling precise kinetic studies and high-throughput screening assays. It is an essential tool for researchers and quality control laboratories in the life sciences, biotechnology, and drug discovery sectors.

Application

• Protease Activity Assays: Primary substrate for fluorometrically measuring chymotrypsin, cathepsin G, and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Enzyme Kinetics Studies: Enables the determination of kinetic parameters (Km, Vmax, kcat) for detailed enzymatic characterization.
• Biochemical Research: A key reagent for studying protease function, regulation, and signaling pathways in cellular processes.
• Diagnostic Development: Serves as a core component in developing fluorogenic diagnostic tests for conditions involving aberrant protease activity.
• Quality Control in Biologics: Used to monitor and validate the activity and purity of enzyme preparations in manufacturing.

Basic Information

Product Name	Suc-Leu-Tyr-Amc
CAS No.	94367-20-1
Molecular Formula	C26H30N4O7
Molecular Weight	510.54 g/mol
Synonyms	Succinyl-Leucyl-Tyrosyl-7-Amido-4-Methylcoumarin; Suc-Leu-Tyr-AMC; Suc-L-Y-AMC; N-Succinyl-L-leucyl-L-tyrosine 7-amido-4-methylcoumarin; Protease Substrate Suc-Leu-Tyr-AMC; Fluorogenic Peptide Substrate for Chymotrypsin; Chymotrypsin Substrate I (AMC); AMC-labeled protease substrate
EINECS	Contact for details

Quality Control

Our Suc-Leu-Tyr-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by mass spectrometry (MS) and infrared spectroscopy (IR). We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications for consistent, reliable performance in sensitive assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	≥ 95% relative activity vs. control

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.