Description

7-Lysylalanyl-4-Methylcoumarinamide is a synthetic peptide-conjugated fluorogenic substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of specific protease activity. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for enzyme kinetics studies, inhibitor screening, and biochemical research.

Application

• Protease Activity Assays: Fluorogenic substrate for the sensitive detection and kinetic analysis of specific proteolytic enzymes.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors.
• Biochemical Research: A key tool for studying enzyme mechanisms, substrate specificity, and catalytic efficiency in vitro.
• Diagnostic Development: Serves as a core component in developing clinical diagnostic kits for detecting enzyme-related pathologies.
• Quality Control (QC) Testing: Employed in pharmaceutical QC to monitor the activity and consistency of enzyme-based products or processes.

Basic Information

Product Name	7-Lysylalanyl-4-Methylcoumarinamide
CAS No.	94149-28-7
Molecular Formula	C24H34N6O6
Molecular Weight	502.57 g/mol
Synonyms	Lys-Ala-AMC; L-Lysyl-L-alanine 7-amido-4-methylcoumarin; AMC Substrate Lys-Ala; 7-(Nε-L-Lysyl-L-alanylamido)-4-methylcoumarin; Fluorogenic Peptide Substrate Lys-Ala; Kallikrein Substrate; K-AMC; Peptide-AMC Conjugate
EINECS	Contact for details

Quality Control

Our 7-Lysylalanyl-4-Methylcoumarinamide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, mass spectrometry for identity confirmation, and fluorometric validation for functional activity. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity (Fluorometric Assay)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.