Description

Nuclease, Bal31 (Alteromonas espejiana) is a highly specific exo- and endonuclease enzyme derived from the marine bacterium *Alteromonas espejiana*. This product is essential for precise DNA manipulation, offering controlled digestion from both the 3' and 5' termini of double-stranded DNA. It is a critical reagent for molecular biology researchers and biotech companies requiring advanced nucleic acid analysis, mapping, and mutagenesis. Its primary value lies in its ability to generate progressive deletions, making it indispensable for structural studies and functional genomics.

Application

• DNA End-Trimming and Deletion Mapping: For controlled removal of nucleotides from DNA ends to create nested deletions for sequencing and functional analysis.
• Site-Directed Mutagenesis: Used to create unidirectional deletions from restriction sites, facilitating the generation of specific mutations.
• Nuclease Protection Assays: Employed to analyze DNA-protein interactions and determine the boundaries of protein-binding sites.
• Molecular Cloning and Vector Construction: For precise modification of DNA fragment ends to ensure correct ligation and cloning efficiency.
• Genomic DNA Analysis: In the characterization of chromatin structure and the analysis of DNA modifications.
• Biotechnology and Pharmaceutical R&D: A key tool in the development of gene therapies, diagnostic kits, and recombinant protein production platforms.

Basic Information

Product Name	Nuclease, Bal31 (Alteromonas espejiana)
CAS No.	94047-22-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Bal 31 Nuclease; BAL-31 Nuclease; Nuclease BAL 31; EC 3.1.11.-; Exodeoxyribonuclease VII; Bacteriophage-associated lysin; Alteromonas espejiana Bal31 nuclease; Deoxyribonuclease (ATP-dependent); ATP-dependent deoxyribonuclease
EINECS	Contact for details

Quality Control

Our Nuclease Bal31 is produced under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high purity, specific activity, and the absence of contaminating nucleases (e.g., RNase, phosphatase). Specifications are aligned with standards for molecular biology-grade enzymes. A comprehensive Certificate of Analysis (COA) detailing activity units, purity, and buffer composition is provided with every shipment to ensure traceability and performance consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in the supplied storage buffer. Avoid repeated freeze-thaw cycles. Aliquotting upon receipt is recommended for frequent use to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Conforms
Specific Activity	≥ 5,000 units/mL
Purity (SDS-PAGE)	≥ 95%
Contaminating Nucleases	Not detected
Contaminating Phosphatase	Not detected
Endotoxin Level	< 0.1 EU/µg (if applicable)
Buffer	50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 50% Glycerol, pH 8.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.