Description

4-Nitrophenyl 3-o-(A-D-Mannopyranosyl)-A-D-Mannopyranoside is a specialized synthetic glycoside derivative, serving as a chromogenic substrate for the detection and kinetic study of α-mannosidase and related glycosidase enzymes. Its core value lies in providing a reliable, colorimetric readout for enzymatic activity, which is critical for biochemical research and diagnostic assay development. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical R&D, and in vitro diagnostics (IVD) sectors who require precise tools for enzyme characterization and inhibitor screening.

Application

• Enzymology Research: Used as a specific chromogenic substrate for the assay of α-mannosidase activity, facilitating kinetic studies and enzyme characterization.
• Diagnostic Reagent Development: Incorporated into clinical diagnostic kits for the detection of genetic disorders related to glycosidase deficiencies, such as certain lysosomal storage diseases.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential glycosidase inhibitors for therapeutic development.
• Biochemical Tool: Serves as a critical reagent in studying carbohydrate metabolism, glycoprotein processing, and quality control pathways within the endoplasmic reticulum and Golgi apparatus.
• Quality Control in Bioprocessing: Used to monitor glycosidase activity during the production and purification of recombinant glycoprotein therapeutics.

Basic Information

Product Name	4-Nitrophenyl 3-o-(A-D-Mannopyranosyl)-A-D-Mannopyranoside
CAS No.	93979-06-7
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	p-Nitrophenyl 3-O-α-D-Mannopyranosyl-α-D-mannopyranoside; 4-Nitrophenyl 3-O-α-D-mannopyranosyl-α-D-mannopyranoside; PNPMann(α1-3)Manα-pNP; α(1-3) Mannobiose p-nitrophenyl glycoside; 3-O-α-D-Mannopyranosyl-α-D-mannopyranoside 4-nitrophenyl; α-D-Manp-(1→3)-α-D-Manp-O-pNP
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 3-o-(A-D-Mannopyranosyl)-A-D-Mannopyranoside is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for sensitive biochemical applications. A detailed Certificate of Analysis (COA) is provided with each shipment, guaranteeing traceability and consistent performance for your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption, which could affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.