Description

5-Bromouridine-5'-Triphosphate Lithium Salt is a modified nucleotide triphosphate analog essential for advanced molecular biology and biochemistry research. This compound serves as a critical substrate for the enzymatic synthesis of RNA molecules containing brominated uracil bases, enabling studies on RNA structure, function, and protein-RNA interactions. It is primarily utilized by researchers in pharmaceutical development, academic institutions, and diagnostic kit manufacturers focused on nucleic acid labeling, cross-linking experiments, and structural probing.

Application

• RNA Synthesis & Labeling: Used as a substrate for in vitro transcription with T7, SP6, or other RNA polymerases to incorporate bromine atoms for heavy-atom phasing in X-ray crystallography.
• Structural Biology Studies: Enables the preparation of bromine-labeled RNA for structural analysis via X-ray diffraction and cryo-electron microscopy (cryo-EM).
• Photo-Crosslinking & Probing: The bromine atom can facilitate UV-induced cross-linking to study RNA-protein interactions or serve as a site for further chemical modification.
• Biochemical Assay Development: Employed in the development of high-throughput screening assays for RNA-binding proteins and antiviral drug discovery targeting viral polymerases.
• Next-Generation Sequencing (NGS): Potential use in the synthesis of specialized nucleotides for novel sequencing methodologies and probe development.

Basic Information

Product Name	5-Bromouridine-5'-Triphosphate Lithium Salt
CAS No.	93882-11-2
Molecular Formula	C9H12BrLiN2O14P3 (commonly represented for the lithium salt form)
Molecular Weight	Contact for details
Synonyms	5-Br-UTP Li Salt; BrUTP Lithium Salt; 5-Bromouridine Triphosphate Lithium Salt; 5-Bromo-UTP; 1-((2R,3S,4R,5R)-5-(6-Amino-5-bromo-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl) 1-(hydroxy({[hydroxy(phosphonooxy)phosphoryl]oxy})phosphoryl) lithium salt; 5-Bromouridine 5'-(tetrahydrogen triphosphate) lithium salt; Bromouridine triphosphate
EINECS	Contact for details

Quality Control

Our 5-Bromouridine-5'-Triphosphate Lithium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤5.0%
Solubility	Soluble in water, slightly soluble in ethanol
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.