Description

5-Bromo-6-Chloro-3-Indoxyl-β-D-Glucopyranoside is a specialized chromogenic substrate used in enzymatic detection systems. This compound is critical for high-sensitivity assays in molecular biology and diagnostic applications, where it serves as a precursor to an insoluble, colored precipitate upon enzymatic cleavage. It is primarily needed by manufacturers of diagnostic kits, research laboratories in life sciences, and companies developing cell-based assays for microbiology and histochemistry.

Application

• Chromogenic Substrate for β-Glucosidase: Used as a key reagent in enzymatic assays to detect and quantify β-glucosidase activity, producing a distinctive blue-green indigo dye.
• Microbiological Testing: Employed in culture media and diagnostic kits for the specific identification of microorganisms, such as E. coli and other coliforms, based on glucosidase activity.
• Molecular Biology Research: Utilized in reporter gene assays (e.g., lacZ gene expression) and in situ hybridization techniques to visualize gene expression patterns in cells and tissues.
• Histochemistry and Cell Staining: Applied in tissue sections and cell cultures to localize enzymatic activity, providing spatial information in biomedical research.
• Diagnostic Kit Manufacturing: A vital raw material for producing test strips, plates, and automated system reagents used in clinical and food safety diagnostics.
• Enzyme Characterization: Serves as a specific substrate for studying the kinetics and inhibition of β-glucosidase enzymes in biochemical research.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indoxyl-β-D-Glucopyranoside
CAS No.	93863-89-9
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl-β-D-glucopyranoside; X-Gluc (5-Bromo-6-Chloro); BCIG; 5-Bromo-6-chloro-1H-indol-3-yl β-D-glucopyranoside; Magenta-Gluc; 93863-89-9; 5-Br-6-Cl-3-indoxyl-β-D-glucoside
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indoxyl-β-D-Glucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.