Description

Succinimidyl-2-(Bromoacetamido)Acetate is a highly reactive heterobifunctional crosslinking agent, specifically engineered for the controlled conjugation of biomolecules. Its core value lies in enabling the formation of stable amide bonds with primary amines via its NHS ester, while the bromoacetamide group provides a unique handle for thiol-specific alkylation or further functionalization. This compound is essential for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and advanced proteomics, where precise and stable linker chemistry is critical.

Application

• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a key intermediate for constructing cleavable or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Labeling: Used to attach fluorescent dyes, biotin, or other probes to proteins via amine groups, with the bromoacetamide allowing for secondary modification or site-specific conjugation.
• Surface Immobilization: Facilitates the covalent attachment of proteins, antibodies, or other ligands to solid supports (e.g., biosensor chips, beads, plates) for assay development.
• Polymer and Biomaterial Functionalization: Enables the modification of amine-containing polymers or hydrogels to introduce reactive bromoacetamide groups for subsequent thiol-click chemistry.
• Crosslinking Studies: Employed in structural biology to study protein-protein interactions or map molecular distances through the introduction of specific crosslinks.
• Diagnostic Reagent Synthesis: A critical building block in the production of enzyme conjugates and other reagents used in immunoassays and diagnostic kits.

Basic Information

Item	Detail
Product Name	Succinimidyl-2-(Bromoacetamido)Acetate
CAS No.	93801-73-1
Molecular Formula	C8H9BrN2O5
Molecular Weight	293.07 g/mol
Synonyms	2-[(Bromoacetyl)amino]acetic acid N-hydroxysuccinimide ester; SBA; NHS-Bromoacetate; Bromoacetic acid N-succinimidyl ester; N-Succinimidyl bromoacetate; N-(Bromoacetyl)-N'-(succinimidyl)glycine; SGBA; Bromoacetamidoacetic acid succinimidyl ester
EINECS	Contact for details

Quality Control

Our Succinimidyl-2-(Bromoacetamido)Acetate is manufactured under strict quality systems to ensure batch-to-batch consistency and high reactivity. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable, ensuring the material is suitable for research and production-scale applications in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent hydrolysis of the active NHS ester. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.