Description

n-Biotinyl-N'-(Iodoacetyl)-1,6-Hexanediamine is a heterobifunctional crosslinking reagent designed for precise bioconjugation. This compound matters because it enables the site-specific labeling of biomolecules, combining the high-affinity biotin-streptavidin interaction with a reactive iodoacetyl group for thiol targeting. Researchers and manufacturers in the fields of proteomics, antibody-drug conjugate (ADC) development, and diagnostic assay fabrication require this reagent to create stable, functionalized complexes for analysis and therapeutic applications.

Application

• Antibody-Drug Conjugate (ADC) Linker: For constructing stable, cleavable linkages between cytotoxic payloads and monoclonal antibodies.
• Protein and Peptide Labeling: Site-specific biotinylation of cysteine-containing proteins for purification (streptavidin affinity) or detection.
• Diagnostic Assay Development: Immobilization of capture antibodies or antigens on streptavidin-coated surfaces (e.g., ELISA, lateral flow assays).
• Affinity Probe Construction: Creating biotin-tagged chemical probes for target identification and pull-down assays in proteomics.
• Surface Plasmon Resonance (SPR) & Biosensor Fabrication: For the oriented immobilization of ligands on sensor chips to study biomolecular interactions.
• Nucleic Acid Conjugation: Labeling thiol-modified oligonucleotides for detection and microarray applications.
• Cell Surface Engineering: Modifying cell surface thiol groups for tracking, sorting, or targeted delivery studies.
• Materials Science: Functionalizing nanoparticles or polymers with biotin for creating smart biomaterials and drug delivery systems.

Basic Information

Product Name	n-Biotinyl-N'-(Iodoacetyl)-1,6-Hexanediamine
CAS No.	93285-75-7
Molecular Formula	C18H31IN4O3S
Molecular Weight	510.43 g/mol
Synonyms	Biotin-HPDP; Biotinylated HPDP; 1,6-Hexanediamine, N-biotinyl-N'-(iodoacetyl)-; N-Biotinyl-N'-(iodoacetyl)-1,6-hexanediamine; Biotin-HPDP Crosslinker; Iodoacetyl-LC-Biotin; EZ-Link Iodoacetyl-LC-Biotin; Biotin-(6-(iodoacetamido)hexyl)amide
EINECS	Contact for details

Quality Control

Our n-Biotinyl-N'-(Iodoacetyl)-1,6-Hexanediamine is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and development. Each lot undergoes rigorous analytical testing. Certificates of Analysis (COA) detailing purity, identity, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.