Description

Pyrenedodecanoyl-Coenzyme A is a specialized, fluorescently-labeled Coenzyme A derivative. This compound is a critical biochemical tool for studying lipid metabolism, enzyme kinetics, and acyltransferase activity. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic developers for high-sensitivity assays and mechanistic studies.

Application

• Enzyme Activity Assays: As a fluorescent substrate for studying acyl-CoA synthetases, acyltransferases, and other enzymes involved in fatty acid metabolism.
• Lipid Metabolism Research: Tracing and quantifying the incorporation of fatty acids into complex lipids within cellular and in vitro systems.
• High-Throughput Screening (HTS): Enabling fluorescence-based screening for drug discovery targets related to metabolic disorders.
• Diagnostic Development: Serving as a key component in developing assays for metabolic enzyme deficiencies.
• Biochemical Probe: Used in mechanistic studies to visualize and monitor protein-lipid interactions and transport processes.
• Academic Research: Fundamental research in biochemistry and molecular biology to elucidate pathways of lipid biosynthesis and degradation.

Basic Information

Product Name	Pyrenedodecanoyl-Coenzyme A
CAS No.	93255-33-5
Molecular Formula	C42H62N7O17P3S
Molecular Weight	1049.96 g/mol
Synonyms	1-Pyrenedodecanoyl-CoA; 12-(1-Pyrenyl)dodecanoyl-CoA; Pyrene-labeled dodecanoyl coenzyme A; N-[(12-Pyren-1-yldodecanoyl)sulfanyl]ethanaminyl 3-[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoate; Fluorescent C12-CoA; P12-CoA
EINECS	Contact for details

Quality Control

Our Pyrenedodecanoyl-Coenzyme A is produced under stringent conditions to ensure high purity and batch-to-batch consistency for research applications. Each lot undergoes comprehensive analysis including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and stability data.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is strictly light-sensitive and hygroscopic. For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Extinction Coefficient (ε)	~42,000 cm-1M-1 at 342 nm (in methanol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.