Description

Sulfo-SMPB is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a sulfhydryl-reactive maleimide group, connected by a spacer arm containing a sulfonate group for enhanced water solubility. This compound is essential for creating stable, covalent conjugates between proteins, peptides, or other amine-containing molecules and thiol-containing compounds under mild, aqueous conditions. It is a critical tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, diagnostic assay development, and surface immobilization for biosensors.

Application

• Antibody-Drug Conjugate (ADC) Linker: Conjugating cytotoxic drugs to monoclonal antibodies via lysine residues.
• Protein-Protein/Peptide Conjugation: Creating stable complexes for structural studies, immunoassays, or multivalent therapeutics.
• Enzyme and Protein Immobilization: Covalently attaching biomolecules to thiol-functionalized surfaces, beads, or chips for biosensors and affinity purification.
• Fluorescent or Biotin Labeling: Introducing tags to proteins or antibodies for detection and imaging applications.
• Hapten-Carrier Protein Conjugation: Preparing immunogens for antibody production in diagnostic and therapeutic research.
• Polymer Modification: Functionalizing polymers with bioactive molecules for drug delivery systems or biomaterials.

Basic Information

Product Name	Sulfo-SMPB
CAS No.	92921-26-1
Molecular Formula	C16H15NO9S2 • Na
Molecular Weight	460.41 g/mol (Sodium salt)
Synonyms	Sulfosuccinimidyl 4-(p-maleimidophenyl)butyrate; Sodium salt of Sulfo-SMPB; 4-Sulfosuccinimidyl 4-(4-maleimidophenyl)butanoate; Sulfo-SMPB Sodium Salt; SMPB Sulfonate; Water-soluble SMPB; Heterobifunctional crosslinker sulfo-SMPB; NHS-PEG4-Maleimide sulfonate analog
EINECS	Contact for details

Quality Control

Our Sulfo-SMPB is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, IR), to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, solubility, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR/IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Freely soluble in water, PBS buffer
Functional Activity	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.