Description

Sulfo-SMCC is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a sulfhydryl-reactive maleimide group, with a water-solubilizing sulfonate group. This compound is essential for creating stable, non-reversible conjugates between proteins, peptides, and other biomolecules, enabling advanced research and diagnostic applications. It is a critical tool for scientists and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and immunoassay production, where controlled and efficient linking of biomolecules is required.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key reagent for covalently attaching cytotoxic drugs to monoclonal antibodies via lysine residues.
• Protein-Protein/Peptide Conjugation: Facilitates the crosslinking of different proteins or peptides to create novel complexes for structural studies or functional assays.
• Enzyme Immobilization: Used to attach enzymes to solid supports, nanoparticles, or other matrices while maintaining activity, for biosensor and diagnostic kit development.
• Immunoassay Reagent: Employed in the preparation of enzyme-antibody or hapten-carrier protein conjugates for ELISA and other immunoassay formats.
• Biomolecule Labeling: Conjugates fluorescent dyes, biotin, or other probes to proteins via available amine groups for detection and imaging.
• Stable Complex Formation: Creates permanent linkages in protein-nucleic acid or protein-small molecule complexes for therapeutic and research applications.

Basic Information

Product Name	Sulfo-SMCC
CAS No.	92921-24-9
Molecular Formula	C14H15NO8S
Molecular Weight	357.34 g/mol
Synonyms	Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate; 4-Sulfo-2,3,5,6-tetrafluorophenyl (STP) ester; Sulfo-SMCC Crosslinker; N-Succinimidyl-4-(maleimidomethyl)cyclohexanecarboxylate, sulfonate; SMCC, sulfonated; Water-soluble SMCC; Heterobifunctional crosslinker sulfo-SMCC; 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
EINECS	Contact for details

Quality Control

Our Sulfo-SMCC is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency for critical research and bioprocessing applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance-related specifications. We support compliance with cGMP and ISO 9001 standards for traceability and quality assurance.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO
Functional Activity	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.