Description

Z-Phe-Cit-Amc is a specialized fluorogenic peptide substrate designed for the detection and quantification of protease activity. This compound is critical for biochemical research and pharmaceutical development, providing a sensitive and reliable tool for enzyme kinetic studies. It is primarily utilized by researchers and quality control laboratories in the life sciences, pharmaceutical, and biotechnology industries for high-throughput screening and diagnostic assay development.

Application

• Protease Activity Assays: A standard fluorogenic substrate for measuring the activity of various proteases, including cathepsins and caspases, in enzymatic studies.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential protease inhibitors for therapeutic development.
• Biochemical Research: Essential for investigating protease function, kinetics, and inhibition mechanisms in cell biology and pathology research.
• Diagnostic Kit Development: Employed in the formulation of diagnostic assays and kits for detecting specific protease biomarkers associated with diseases.
• Quality Control in Biologics: Applied in the quality control testing of biopharmaceuticals to monitor and ensure the absence of residual protease activity.

Basic Information

Product Name	Z-Phe-Cit-Amc
CAS No.	92745-52-3
Molecular Formula	C30H38N4O7
Molecular Weight	566.65 g/mol
Synonyms	Z-Phe-Cit-AMC; Z-Phe-Cit-7-amido-4-methylcoumarin; Benzyloxycarbonyl-Phe-Cit-AMC; Z-Phe-Cit-AFC (common alternate); Caspase Substrate; Cathepsin L Substrate; Fluorogenic Peptide Substrate Z-FC-Amc; N-CBZ-Phenylalanyl-Citrulline-7-Amido-4-Methylcoumarin
EINECS	Contact for details

Quality Control

Our Z-Phe-Cit-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.