Description

4-Methylumbelliferyl -Chitotetraose Tridecaacetate is a specialized fluorogenic substrate designed for the sensitive detection and quantification of chitinase and lysozyme enzyme activity. This compound matters as a critical research tool, enabling precise biochemical analysis in enzymatic studies due to its specific cleavage by target enzymes to release a highly fluorescent product. It is primarily needed by researchers and quality control laboratories in the life sciences, pharmaceutical development, and agricultural biotechnology sectors for studying chitin degradation pathways and developing enzyme-based assays.

Application

• Enzyme Activity Assays: Primary substrate for the fluorometric determination of endo-chitinase, exo-chitinase, and lysozyme activity in research and diagnostic applications.
• Biochemical Research: Used in kinetic studies, inhibitor screening, and characterization of enzyme specificity and mechanism of action.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify potential antifungal or antibacterial drug candidates that target chitin metabolism.
• Agricultural Biotechnology: Applied in research on plant-pathogen interactions and the development of transgenic crops with enhanced fungal resistance.
• Diagnostic Kit Manufacturing: Serves as a key component in the formulation of commercial kits for measuring chitinase levels in clinical or environmental samples.
• Quality Control: Used as a reference standard in the quality assurance of enzyme preparations and related bioproducts.

Basic Information

Product Name	4-Methylumbelliferyl -Chitotetraose Tridecaacetate
CAS No.	92574-74-8
Molecular Formula	C63H73NO38
Molecular Weight	1476.24 g/mol
Synonyms	4-MU-(GlcNAc)4 Tridecaacetate; 4-Methylumbelliferyl β-D-N,N',N'',N'''-tetraacetylchitotetraoside; 4-MU-Chitotetraose 13-acetate; Fluorescent Chitotetraose Substrate; 4-Methylumbelliferyl N,N',N'',N'''-tetraacetylchitotetraoside; Chitinase Fluorogenic Substrate IV; Lysozyme Substrate (Fluorogenic)
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl -Chitotetraose Tridecaacetate is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) detailing all analytical results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.