Description

5-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Pentyl Methylphosphonofluoridate is a specialized organophosphorus compound featuring a reactive phosphonofluoridate group coupled with a fluorescent NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) reporter. This unique structure makes it a critical intermediate for synthesizing active site-directed probes and inhibitors for studying serine hydrolases, including acetylcholinesterase and related enzymes. It is primarily utilized by research institutions and pharmaceutical R&D departments engaged in neurobiology, toxicology, and the development of diagnostic assays. The compound is supplied with a CAS number of 92457-51-7.

Application

• Biochemical Research: Key intermediate for the synthesis of fluorescent or affinity-labeled organophosphorus agents for studying enzyme kinetics and mechanisms.
• Proteomics & Target Identification: Used to create activity-based probes (ABPs) for the profiling and identification of serine hydrolases in complex biological samples.
• Neuroscience Studies: Enables the development of tools to visualize and quantify acetylcholinesterase activity and localization in neural tissues.
• Diagnostic Assay Development: Serves as a precursor for reagents used in high-throughput screening and diagnostic tests for exposure to organophosphorus compounds.
• Chemical Biology Tool Development: Employed in the design of covalent inhibitors and molecular probes for functional proteomics and drug discovery campaigns.
• Toxicology Research: Used to model and investigate the biochemical pathways of organophosphate toxicity and detoxification.

Basic Information

Item	Details
Product Name	5-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Pentyl Methylphosphonofluoridate
CAS No.	92457-51-7
Molecular Formula	C₁₃H₁₇FN₃O₆P
Molecular Weight	361.26 g/mol
Synonyms	NBD-C5-Methylphosphonofluoridate; 5-(NBD-amino)pentyl methylphosphonofluoridate; Methylphosphonofluoridic Acid 5-[(7-Nitro-4-benzofurazanyl)amino]pentyl Ester; 5-(7-Nitro-2,1,3-benzoxadiazol-4-yl)aminopentyl methylphosphonofluoridate; NBD-AP-MP-F; Fluorescent organophosphorus agent; Serine hydrolase probe intermediate
EINECS	Contact for details

Quality Control

Every batch of 5-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Pentyl Methylphosphonofluoridate is synthesized and purified under controlled conditions to ensure high chemical integrity and lot-to-lot consistency. Our quality assurance includes rigorous analytical testing via HPLC, NMR (¹H, ³¹P), and mass spectrometry to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at or below -20°C under an inert atmosphere (e.g., argon or nitrogen) due to its moisture-sensitive (hygroscopic) nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure (¹H, ³¹P)
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.