Description

Bis(Benzyloxycarbonylisoleucyl-Propyl-Argininamide)Rhodamine is a specialized, fluorescently labeled peptide derivative designed for advanced biochemical research and diagnostic applications. This compound is critical for researchers requiring high-precision, sensitive detection and quantification in complex biological systems. It is primarily utilized by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic institutions focused on protease activity studies, fluorescence resonance energy transfer (FRET) assays, and cellular imaging.

Application

• Protease & Enzyme Activity Assays: Serves as a highly sensitive fluorogenic substrate for monitoring the activity of specific proteases, including trypsin-like enzymes.
• FRET-Based Research: Acts as a key component in Fluorescence Resonance Energy Transfer (FRET) systems for studying protein-protein interactions and conformational changes in real-time.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery for screening libraries of compounds against specific enzymatic targets in automated assay platforms.
• Diagnostic Development: Incorporated into the development of clinical diagnostic tests and kits for detecting enzymatic activity related to specific disease states.
• Biochemical Pathway Analysis: Enables the visualization and quantification of specific biochemical pathways within live cells or tissue samples.
• Cell Biology & Imaging: Utilized as a fluorescent probe for intracellular localization studies and tracking enzymatic processes using fluorescence microscopy.

Basic Information

Product Name	Bis(Benzyloxycarbonylisoleucyl-Propyl-Argininamide)Rhodamine
CAS No.	91847-26-6
Molecular Formula	C66H86N14O13
Molecular Weight	1259.5 g/mol
Synonyms	Bis(Z-Ile-Pro-Arg) Rhodamine 110; Z-Ile-Pro-Arg-R110; Bis-CBZ-Ile-Pro-Arg-Rhodamine; Fluorogenic Substrate for Trypsin; Rhodamine 110, bis-(N-CBZ-Ile-Pro-Arg amide); FRET Substrate R110; Protease Substrate R110; Boc-Ile-Pro-Arg-R110 (common abbreviation); CBZ-Ile-Pro-Arg-R110
EINECS	Contact for details

Quality Control

Every batch of Bis(Benzyloxycarbonylisoleucyl-Propyl-Argininamide)Rhodamine is manufactured and tested under strict quality management systems to ensure exceptional purity and performance consistency for sensitive research applications. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. Our quality commitment aligns with the standards expected for high-grade biochemical reagents used in pharmaceutical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions, light, or moisture will lead to degradation. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95% (Area Percent)
Assay (Fluorometric)	≥ 90% (Enzymatic activity vs. reference)
Water Content (KF)	≤ 2.0 %
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.