Description

Methotrexate CAS NO 91431-42-4 is a potent antimetabolite and antifolate agent, widely recognized for its critical role in chemotherapy and immunosuppressive therapy. Its primary value lies in its ability to inhibit dihydrofolate reductase, effectively disrupting DNA synthesis and cellular replication. This makes it an essential active pharmaceutical ingredient (API) for manufacturers in the pharmaceutical industry, particularly for the treatment of various cancers, autoimmune diseases like rheumatoid arthritis, and severe psoriasis.

Application

• Oncological Therapeutics: A cornerstone chemotherapeutic agent used in the treatment of leukemia, lymphoma, breast cancer, lung cancer, and osteosarcoma.
• Autoimmune Disease Management: Key immunosuppressive component in formulations for rheumatoid arthritis, psoriasis, and Crohn's disease.
• Pharmaceutical Intermediates: Serves as a critical starting material or intermediate in the synthesis of more complex pharmaceutical compounds and targeted therapies.
• Research & Development: Used in biochemical and pharmacological research to study folate metabolism, cell proliferation, and mechanisms of immunosuppression.
• Dermatological Treatments: Employed in topical or systemic treatments for severe, recalcitrant psoriasis.
• Ectopic Pregnancy Treatment: Used as a non-surgical treatment option for certain cases of ectopic pregnancy.

Basic Information

Item	Details
Product Name	Methotrexate
CAS No.	91431-42-4
Molecular Formula	C₂₀H₂₂N₈O₅
Molecular Weight	454.44 g/mol
Synonyms	Methotrexate; Amethopterin; MTX; 4-Amino-10-methylfolic acid; 4-Amino-10-methylpteroylglutamic acid; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-; Rheumatrex; Trexall
EINECS	Contact for details

Quality Control

Our Methotrexate is manufactured under strict quality management systems to ensure it meets the rigorous standards required for pharmaceutical applications. Each batch undergoes comprehensive analytical testing, including identification, assay, impurity profiling, and residual solvent analysis, typically via HPLC, IR, and other pharmacopeial methods. We provide full traceability and Certificates of Analysis (COA) are available for every shipment, confirming compliance with relevant specifications such as USP, EP, or customer-defined requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature, typically between 15°C and 25°C (59°F and 77°F). The product is light-sensitive and should be handled and stored accordingly to maintain stability and potency. Keep the container in a dry, well-ventilated area.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to standard
Identification (HPLC)	Conforms to standard
Assay (HPLC)	98.0% - 102.0% (on anhydrous basis)
Water Content (KF)	≤ 12.0%
Related Substances (HPLC)	Individual impurity: ≤ 0.5% Total impurities: ≤ 2.0%
Residual Solvents (GC)	Complies with ICH Q3C guidelines
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.