Description

5-Phosphoribosyl 1-o-(1-Thiodiphosphate) is a high-purity biochemical reagent, a sulfur-containing analog of the key metabolic intermediate 5-phosphoribosyl 1-pyrophosphate (PRPP). This compound is of significant value as a stable, non-hydrolyzable substrate analog for enzymatic studies and inhibitor research. It is primarily utilized by research institutions and pharmaceutical companies engaged in nucleotide metabolism, enzyme kinetics, and the development of novel therapeutic agents targeting purine and pyrimidine biosynthesis pathways.

Application

• Enzymology Research: Acts as a critical substrate analog for studying enzymes in the purine and pyrimidine salvage pathways, such as phosphoribosyltransferases.
• Drug Discovery & Development: Serves as a valuable tool compound for screening and characterizing inhibitors targeting nucleotide metabolism, a strategy in anticancer and antimicrobial drug design.
• Biochemical Assay Development: Used in the development of high-throughput screening (HTS) assays to identify modulators of PRPP-utilizing enzymes.
• Metabolic Pathway Analysis: Employed in in vitro studies to elucidate and map complex nucleotide biosynthetic and salvage pathways.
• Academic & Institutional Research: Essential for fundamental biochemical research in universities and government labs focusing on cellular metabolism and enzymology.

Basic Information

Product Name	5-Phosphoribosyl 1-o-(1-Thiodiphosphate)
CAS No.	91389-14-9
Molecular Formula	C5H13O13P3S
Molecular Weight	406.11 g/mol
Synonyms	PRPP-S; 5-Phosphoribosyl 1-Thiodiphosphate; 5-Phospho-α-D-ribofuranosyl 1-thiodiphosphate; 1-Thio-5-phosphoribosyl diphosphate; α-5-Phosphoribosyl 1-thiodiphosphate; P1-(5-Phospho-α-D-ribosyl) P2-thiodiphosphate; Thiophosphoribosyl pyrophosphate; PRPP analog (thio)
EINECS	Contact for details

Quality Control

Our 5-Phosphoribosyl 1-o-(1-Thiodiphosphate) is manufactured under stringent conditions to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A detailed Certificate of Analysis (COA) providing purity, assay, and impurity profiles is supplied with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by enzymatic or titration method)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or suitable buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.