Description

Uridine Diphosphate Galactose, [Galactose-6-3H] is a high-specific-activity radiolabeled nucleotide sugar derivative, essential for tracing and quantifying galactose metabolism in biological systems. This tritiated compound is a critical reagent for researchers investigating glycosylation pathways, enzyme kinetics, and the biosynthesis of glycoproteins and glycolipids. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic assay development where precise metabolic tracking is required.

Application

• Enzyme Assay Substrate: Used as a labeled substrate for assays of galactosyltransferases, including β-1,4-galactosyltransferase (β4GalT), to study enzyme activity and inhibition.
• Metabolic Pathway Tracing: Enables the detailed study of the Leloir pathway and other galactose utilization routes in cell culture and in vitro systems.
• Glycoconjugate Biosynthesis Research: Critical for radiolabeling studies investigating the biosynthesis of N-linked and O-linked glycoproteins, proteoglycans, and glycolipids.
• Diagnostic Development: Serves as a key component in the development of radioenzymatic assays for diagnosing inborn errors of metabolism, such as galactosemia.
• Pharmaceutical R&D: Used in drug discovery programs targeting glycosylation disorders, cancer (altered cell surface glycosylation), and infectious diseases.
• Cell Biology Studies: Applied in autoradiography and pulse-chase experiments to visualize and quantify galactose incorporation into cellular macromolecules.

Basic Information

Product Name	Uridine Diphosphate Galactose, [Galactose-6-3H]
CAS No.	90689-99-9
Molecular Formula	C15H24N2O17P2 (Unlabeled Parent Compound)
Molecular Weight	566.31 g/mol (Unlabeled Parent Compound)
Synonyms	UDP-Galactose, [6-3H]; UDP-[6-3H]Galactose; UDP-Gal, [6-3H]; Uridine 5'-(α-D-galactopyranosyl diphosphate), [6-3H]; UDP-α-D-[6-3H]Galactose; Tritiated UDP-Galactose; Galactosyl Donor Substrate, [3H]; Uridine Diphosphogalactose, [6-3H]
EINECS	Contact for details

Quality Control

Every batch of our radiolabeled biochemicals is produced and tested under stringent quality protocols to ensure high radiochemical purity and specific activity. Analytical control includes HPLC (UV and radiometric detection) and TLC to verify identity and purity. A comprehensive Certificate of Analysis (COA) is supplied with each shipment, detailing specific activity, radiochemical purity, chemical purity, and solvent composition. Our quality system supports research requiring reliable and reproducible tracer compounds.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at ≤ -20°C or as recommended on the specific product COA. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture ingress. Store in accordance with local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	15-40 Ci/mmol (Contact for exact value)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC, UV)	≥ 90%
Solvent	Ethanol:Water solution
Concentration	1 mCi/mL (Approx.)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.