Description

Proteasome Substrate Vi, Fluorogenic is a specialized, high-purity fluorogenic peptide designed for the sensitive and continuous measurement of proteasome activity. This compound matters as a critical research tool for studying proteasome function, a key regulator of protein degradation in cellular processes. It is essential for researchers and scientists in the fields of biochemistry, molecular biology, and drug discovery, particularly those investigating cancer, neurodegenerative diseases, and the mechanisms of targeted protein degradation.

Application

• Biochemical Research: Used as a specific substrate for in vitro assays to measure the chymotrypsin-like activity of the 20S proteasome.
• Drug Discovery & Screening: Enables high-throughput screening (HTS) of potential proteasome inhibitors for therapeutic development in oncology and immunology.
• Enzyme Kinetics Studies: Facilitates the determination of kinetic parameters (Km, Vmax) for proteasome enzymatic activity under various conditions.
• Cellular Pathway Analysis: Applied in cell-based assays to monitor proteasome activity and its modulation in response to cellular stress or drug treatment.
• Diagnostic Development: Serves as a core component in developing diagnostic kits for diseases associated with proteasome dysfunction.
• Quality Control: Used in pharmaceutical and biotech manufacturing to test the potency and consistency of proteasome-targeting drug products.

Basic Information

Product Name	Proteasome Substrate Vi, Fluorogenic
CAS No.	90468-18-1
Molecular Formula	C34H45FN4O9
Molecular Weight	672.75 g/mol
Synonyms	Suc-Leu-Leu-Val-Tyr-AMC; N-Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin; Suc-LLVY-AMC; Proteasome Substrate Suc-LLVY-AMC; 20S Proteasome Substrate; Chymotrypsin-like Proteasome Substrate; Fluorogenic Proteasome Substrate VI; N-Succinyl-L-leucyl-L-leucyl-L-valyl-L-tyrosine 7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Proteasome Substrate Vi, Fluorogenic is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to relevant quality standards for research chemicals, ensuring the material is suitable for critical life science and pharmaceutical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate and keep frozen. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity	≥ 90% of reference standard activity in a standard proteasome assay
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.