Description

Z-Leu-Leu-Arg-Amc is a fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound is critical for biochemical research and pharmaceutical development, enabling precise enzymatic studies. It is primarily utilized by researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical industries for high-throughput screening and mechanistic studies of proteolytic enzymes.

Application

• Protease Activity Assays: Fluorogenic substrate for measuring the activity of trypsin-like serine proteases, including thrombin, plasmin, and kallikreins.
• High-Throughput Screening (HTS): Essential tool in drug discovery campaigns to identify and characterize novel protease inhibitors.
• Biochemical Research: Used in enzyme kinetics studies to determine parameters such as Km and Vmax for target proteases.
• Diagnostic Development: Serves as a key component in developing in vitro diagnostic tests for conditions related to abnormal protease activity.
• Cell Biology Studies: Employed to monitor protease activity in cellular and tissue extracts.
• Quality Control in Biologics: Used to assess the purity and activity of enzyme-based pharmaceutical products.

Basic Information

Product Name	Z-Leu-Leu-Arg-Amc
CAS No.	90468-17-0
Molecular Formula	C30H47N7O7
Molecular Weight	617.75 g/mol
Synonyms	Z-Leu-Leu-Arg-AMC; Benzyloxycarbonyl-Leucyl-Leucyl-Arginine 7-Amido-4-Methylcoumarin; Boc-Leu-Leu-Arg-AMC (common alias); Z-LLR-AMC; Fluorogenic Substrate for Trypsin-like Proteases; Nα-CBZ-L-Leucyl-L-Leucyl-L-Arginine 4-Methylcoumaryl-7-Amide; Ac-Leu-Leu-Arg-AMC (related compound); Protease Substrate VI
EINECS	Contact for details

Quality Control

Every batch of Z-Leu-Leu-Arg-Amc is manufactured and tested under strict quality management systems. We ensure high purity and batch-to-batch consistency through comprehensive analytical techniques including HPLC and mass spectrometry. A Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference standard in fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.