Description

Boc-Ala-Ala-Gly-Pna is a protected tripeptide derivative, specifically a p-nitroanilide (Pna) substrate. This compound serves as a critical biochemical tool for the study and characterization of proteolytic enzymes, particularly serine and cysteine proteases. It is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors who require high-purity, well-defined substrates for enzyme kinetics, inhibitor screening, and diagnostic assay development.

Application

• Protease Substrate: A chromogenic substrate for the kinetic analysis and characterization of various proteases, including elastase, cathepsin, and other serine proteases.
• Enzyme Inhibitor Screening: Used in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors for drug discovery programs.
• Biochemical Research: A key reagent in fundamental research to study enzyme mechanisms, specificity, and catalytic activity.
• Diagnostic Kit Development: Employed in the development of colorimetric diagnostic assays and kits that measure protease activity in clinical or research samples.
• Pharmaceutical Intermediate: Can serve as a synthetic intermediate or a model compound in the research and development of peptide-based therapeutics.
• Quality Control in Bioprocessing: Used to monitor and validate the activity of enzyme preparations used in industrial bioprocesses.

Basic Information

Product Name	Boc-Ala-Ala-Gly-Pna
CAS No.	90037-94-8
Molecular Formula	C19H27N5O7
Molecular Weight	437.45 g/mol
Synonyms	Boc-Ala-Ala-Gly-pNA; tert-Butyloxycarbonyl-Alanyl-Alanyl-Glycine p-Nitroanilide; N-(tert-Butoxycarbonyl)-L-alanyl-L-alanylglycine 4-nitroanilide; Boc-AAG-pNA; Ala-Ala-Gly p-nitroanilide, Boc-protected; Protease Substrate Boc-AAG-pNA; Nα-Boc-L-alanyl-L-alanylglycine 4-nitroanilide
EINECS	Contact for details

Quality Control

Our Boc-Ala-Ala-Gly-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C to -10°C. This product is hygroscopic (moisture-sensitive). Allow the sealed container to reach room temperature before opening to prevent condensation and moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.