Description

Nadh-Fmn Oxidoreductase From Vibrio CAS NO 9079-67-8 is a highly specific enzyme that catalyzes the electron transfer between NADH and FMN. This biocatalyst is critical for research and industrial processes requiring precise redox reactions. It is primarily utilized by biochemists, pharmaceutical researchers, and manufacturers in the life sciences sector for enzymatic studies, diagnostic reagent development, and biocatalytic synthesis.

Application

• Biochemical Research & Enzyme Kinetics: Used as a model enzyme for studying electron transfer mechanisms, cofactor interactions, and oxidoreductase activity.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of clinical diagnostic kits, particularly for assays measuring NADH/NAD+ ratios or dehydrogenase activity.
• Pharmaceutical Intermediate Synthesis: Employed in biocatalytic pathways for the stereoselective synthesis of chiral intermediates in active pharmaceutical ingredient (API) development.
• Biosensor Development: Integrated into electrode surfaces or detection systems to create sensitive and specific biosensors for metabolites like lactate or glucose.
• Cofactor Regeneration Systems: Facilitates the in-situ regeneration of expensive coenzymes (NADH/FMN) in enzymatic reaction cascades, improving process economics.
• Microbial Metabolic Pathway Analysis: A tool for investigating and engineering metabolic pathways in Vibrio species and related bacteria for synthetic biology applications.

Basic Information

Product Name	Nadh-Fmn Oxidoreductase From Vibrio
CAS No.	9079-67-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NADH:FMN oxidoreductase; Vibrio NADH dehydrogenase (FMN); NADH-flavin mononucleotide oxidoreductase; EC 1.6.8.1; Old Yellow Enzyme homolog (from Vibrio); FMN-dependent NADH oxidoreductase; Bacterial NADH:flavin oxidoreductase; Vibrio fischeri NADH:FMN oxidoreductase
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is characterized for specific activity, purity, and stability. Certificates of Analysis (COA) detailing assay results, protein concentration, and activity units are available upon request. Production can adhere to relevant guidelines for research-grade enzymes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions when possible. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Catalyzes NADH-dependent reduction of FMN
Specific Activity	≥ [Value] units/mg protein
Protein Content (Bradford)	≥ [Value] %
Purity (SDS-PAGE)	≥ 90%
Solubility	Soluble in appropriate buffer (e.g., Tris, phosphate)
pH Optimum	~7.0 - 8.0
Temperature Optimum	~25 - 37°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.