Description

Alpha2,3-(N)-Sialyltransferase is a highly specific glycosyltransferase enzyme that catalyzes the transfer of sialic acid residues to terminal galactose or N-acetylgalactosamine residues on glycoproteins and glycolipids, forming α2,3 linkages. This enzymatic precision is critical for the synthesis of complex carbohydrate structures essential for biological recognition processes. It is a vital reagent for researchers and manufacturers in the fields of glycobiology research, biopharmaceutical development, and the production of glycoengineered therapeutics and diagnostic tools.

Application

• Glycoprotein Engineering: For the in vitro or cell-based synthesis of sialylated glycoproteins with defined glycan structures to improve therapeutic properties like serum half-life and targeting.
• Biopharmaceutical Development: Used in the production and quality control of monoclonal antibodies, enzymes, and other biologics where specific sialylation patterns are critical for efficacy and safety.
• Glycan Array and Diagnostic Reagent Production: Enzymatic synthesis of defined sialylated oligosaccharides for use in microarray platforms, diagnostic assays, and biomarker discovery.
• Basic Glycobiology Research: A key tool for studying the role of α2,3-linked sialic acids in cell signaling, pathogen recognition, immune response, and cancer metastasis.
• Sialylglycoconjugate Synthesis: For the chemoenzymatic production of sialylated compounds used as standards, probes, or potential therapeutic agents.
• Cell Surface Remodeling: Application in modifying cell surface glycans for studies in stem cell biology, immunology, and tissue engineering.

Basic Information

Product Name	Alpha2,3-(N)-Sialyltransferase
CAS No.	9075-81-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	α-2,3-Sialyltransferase; ST3Gal; CMP-N-acetylneuraminate-β-galactosamide-α-2,3-sialyltransferase; Sialyltransferase 3; β-galactoside α-2,3-sialyltransferase; EC 2.4.99.4; ST3GAL; α-2,3-sialyltransferase
EINECS	Contact for details

Quality Control

Our Alpha2,3-(N)-Sialyltransferase is produced and tested under stringent conditions to ensure high specific activity, purity, and lot-to-lot consistency. Each batch is characterized for enzymatic activity, protein purity, and the absence of contaminating glycosidase activities. A comprehensive Certificate of Analysis (COA) detailing purity, activity (U/mg), buffer composition, and storage conditions is provided with every shipment to support your research and development traceability requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after use. Store in a cool, dry, and well-ventilated area.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Activity)	Catalyzes the formation of α2,3-sialyl linkages
Specific Activity	≥ X U/mg protein (Lot-specific)
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	X mg/mL (Lot-specific)
Buffer Composition	Specified in COA
Preservative	None
Endotoxin Level	< 1.0 EU/μg (for applicable grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.