Description

α-Glycerophosphate Dehydrogenase (α-GPDH) is a critical enzyme that catalyzes the reversible oxidation-reduction reaction between glycerol-3-phosphate and dihydroxyacetone phosphate. This reaction is fundamental to the glycerol phosphate shuttle, a key metabolic pathway for transferring reducing equivalents across mitochondrial membranes. As such, it is an essential reagent for research and diagnostic applications in biochemistry and cell biology. This high-purity enzyme is primarily utilized by the life sciences research sector, pharmaceutical development teams, and in-vitro diagnostic (IVD) kit manufacturers.

Application

• Biochemical Research: Essential for studying mitochondrial energy metabolism, the glycerol phosphate shuttle, and cellular redox states.
• Diagnostic Assays: Used as a key component in enzymatic clinical chemistry kits for the quantitative determination of triglycerides, glycerol, and other metabolites in blood serum.
• Pharmaceutical Development: Employed in enzyme-coupled assays for high-throughput screening (HTS) of drug candidates targeting metabolic disorders.
• Enzyme Kinetics Studies: Serves as a model enzyme for investigating coenzyme (NAD+/NADH) binding, reaction mechanisms, and inhibitor interactions.
• Food & Beverage Analysis: Applied in enzymatic methods for the quality control and analysis of fats, oils, and glycerol content in food products.
• Academic Teaching: Used in undergraduate and graduate laboratory courses to demonstrate principles of enzymology and metabolic pathways.

Basic Information

Product Name	α-Glycerophosphate Dehydrogenase
CAS No.	9075-65-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	α-Glycerophosphate Dehydrogenase; Glycerol-3-phosphate Dehydrogenase (NAD+); sn-Glycerol-3-phosphate Dehydrogenase; α-GPDH; GPDH; G3PDH; L-Glycerol-3-phosphate Dehydrogenase; EC 1.1.1.8
EINECS	Contact for details

Quality Control

Our α-Glycerophosphate Dehydrogenase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is characterized for specific activity, purity, and stability. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as activity units/mg protein, protein concentration, and stability data. Our quality commitment aligns with standards relevant for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can degrade enzymatic activity. Avoid repeated freeze-thaw cycles. For in-use aliquots stored at 2-8°C, stability is limited; refer to the specific COA.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	>100 units/mg protein (Typical)
Protein Content	Contact for details
Purity (SDS-PAGE)	>90%
Stabilizers	May contain buffer salts (e.g., phosphate, Tris) and stabilizers (e.g., BSA, glycerol)
Solubility	Soluble in water or specified buffer
Storage Buffer	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.