Description

Phosphoribosyltransferase, Purine is a critical enzyme that catalyzes the transfer of a phosphoribosyl group, playing an indispensable role in the *de novo* and salvage pathways of purine nucleotide biosynthesis. This enzymatic activity is fundamental for cellular metabolism, making it a valuable reagent for biochemical research and diagnostic applications. It is primarily utilized by researchers in pharmaceutical development, academic institutions, and biotechnology companies focusing on nucleotide metabolism, enzyme kinetics, and targeted therapeutic discovery.

Application

• Biochemical Research & Enzyme Kinetics: Used as a key reagent to study purine metabolism, enzymatic mechanisms, and reaction pathways in vitro.
• Pharmaceutical Development: Employed in screening assays and research for developing inhibitors targeting nucleotide synthesis, relevant for oncology and antiviral drug discovery.
• Diagnostic Kit Manufacturing: Serves as a component in enzymatic diagnostic kits for measuring purine metabolites or related enzyme activities in clinical samples.
• Academic & Life Science Studies: Essential for educational and investigative purposes in molecular biology, biochemistry, and metabolic disorder research.
• Enzyme Production & Standardization: Acts as a reference standard for quality control and activity calibration in the production of other enzymatic products.

Basic Information

Product Name	Phosphoribosyltransferase, Purine
CAS No.	9075-52-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Purine Phosphoribosyltransferase; PPRT; HGPRT (Hypoxanthine-Guanine Phosphoribosyltransferase) related activity; Amidophosphoribosyltransferase (involved in purine synthesis); Glutamine Phosphoribosylpyrophosphate Amidotransferase; ATase; EC 2.4.2.14 (for specific isoforms); Purine nucleotide pyrophosphorylase
EINECS	Contact for details

Quality Control

Our Phosphoribosyltransferase, Purine is produced and tested under stringent conditions to ensure high activity, purity, and lot-to-lot consistency suitable for research and industrial applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing specific activity, purity (via SDS-PAGE or HPLC), and absence of contaminating enzymatic activities. We adhere to relevant quality management standards to guarantee a reliable supply of this critical biochemical reagent.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms (Enzymatic assay)
Specific Activity	> X U/mg protein (Lot-specific)
Purity (SDS-PAGE)	> 90%
Protein Content (Biuret/Bradford)	Lot-specific value provided
pH (in solution)	6.0 - 8.0
Endotoxin (LAL test)	< 1.0 EU/µg (for research grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.