Description

Pyroglutamat Aminopeptidase From E. Coli is a highly purified, recombinant enzyme derived from *Escherichia coli*, specifically designed for the cleavage of N-terminal pyroglutamate residues from peptides and proteins. This enzymatic activity is critical for protein sequencing, structural analysis, and the production of therapeutic biologics where N-terminal pyroglutamate can block Edman degradation. It is an essential tool for biotechnology research, pharmaceutical development, and quality control laboratories requiring precise N-terminal modification or analysis of recombinant proteins and antibodies.

Application

• Protein Sequencing and Characterization: Removal of N-terminal pyroglutamate to enable Edman degradation and mass spectrometric analysis of peptides and proteins.
• Therapeutic Antibody Processing: Cleavage of pyroglutamate from monoclonal antibodies (mAbs) and other biologics to ensure correct N-terminal sequence and homogeneity.
• Recombinant Protein Production: Used in downstream processing to modify or verify the N-terminus of expressed proteins in biomanufacturing.
• Proteomics Research: A key reagent in workflows for identifying and characterizing post-translationally modified proteins.
• Peptide Synthesis and Mapping: Facilitates the analysis and purification of synthetic peptides by cleaving specific blocking groups.
• Diagnostic Kit Component: Incorporated into enzymatic assay kits for research and clinical diagnostics targeting specific protein biomarkers.

Basic Information

Product Name	Pyroglutamat Aminopeptidase From E. Coli
CAS No.	9075-21-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Pyroglutamate Aminopeptidase; Pyroglutamyl Peptidase I; PGP I; 5-Oxoprolyl-peptidase; L-Pyroglutamyl Peptide Hydrolase; Pyrrolidonyl Peptidase; EC 3.4.19.3; Recombinant Pyroglutamate Aminopeptidase; E. coli Pyroglutamyl Aminopeptidase
EINECS	Contact for details

Quality Control

Our Pyroglutamat Aminopeptidase is produced under stringent quality management systems. Each lot undergoes comprehensive analytical testing including activity assays, purity assessment via SDS-PAGE, and absence of contaminating protease activities. A detailed Certificate of Analysis (COA) is provided with each shipment, ensuring traceability and compliance with research-grade specifications. We support customers requiring documentation for regulatory submissions.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquot reconstituted enzyme to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Cleaves N-terminal pyroglutamate from synthetic substrate
Specific Activity	≥ X units/mg protein
Purity (SDS-PAGE)	≥ 95%
Protein Concentration	By UV absorbance
Contaminating Protease Activity	Not detected
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.