Description

β-Lactamase is a critical enzyme that catalyzes the hydrolysis of β-lactam antibiotics, rendering them inactive. This property makes it an essential tool for research and development in combating antibiotic resistance. It is primarily utilized by pharmaceutical manufacturers, diagnostic kit developers, and academic research institutions. The product is supplied as a high-purity, lyophilized powder to ensure optimal activity and stability.

Application

• Antibiotic Resistance Research: Used as a model enzyme to study the mechanisms of β-lactam antibiotic inactivation and to develop novel inhibitors.
• Pharmaceutical Manufacturing: Employed in quality control and process development to degrade residual β-lactam antibiotics in fermentation broths or final products.
• Diagnostic Assays: A key component in diagnostic tests and kits designed to detect β-lactam antibiotic residues in food (e.g., milk, honey) and environmental samples.
• Biochemical Research: Serves as a standard or reagent in enzymatic studies, protein characterization, and molecular biology applications.
• Enzyme Engineering: Used as a starting point for protein engineering efforts aimed at creating enzymes with altered substrate specificity or enhanced stability.

Basic Information

Product Name	β-Lactamase
CAS No.	9073-60-3
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	~29 kDa (Approximate, varies by isoform)
Synonyms	Penicillinase; β-lactamase; Cephalosporinase; EC 3.5.2.6; Penicillin amido-β-lactamhydrolase; β-lactam hydrolase; Penicillin β-lactamase
EINECS	Contact for details

Quality Control

Our β-Lactamase is produced and tested under stringent conditions to ensure high specific activity and purity. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as protein content, specific activity, and purity profile. We adhere to relevant quality management standards to guarantee batch-to-batch consistency and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Upon reconstitution, aliquot and store at -20°C or below to prevent loss of activity. The product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a dry environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Protein Content (Biuret)	≥ 90%
Specific Activity	≥ 1000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
pH (in solution)	6.0 - 8.0
Loss on Drying	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.