Description

Reverse Transcriptase is a critical enzyme that catalyzes the transcription of RNA into complementary DNA (cDNA), a foundational process in molecular biology. This product is essential for researchers and developers requiring high-fidelity and reliable performance in nucleic acid analysis and synthesis. It is primarily utilized in biotechnology, pharmaceutical research, and diagnostic development for applications such as RT-PCR, cDNA library construction, and viral load detection.

Application

• Reverse Transcription Polymerase Chain Reaction (RT-PCR): For the sensitive detection and quantification of RNA transcripts in gene expression analysis and viral diagnostics.
• cDNA Library Construction: Enabling the synthesis of complementary DNA from messenger RNA (mRNA) for genomic and transcriptomic studies.
• Viral Research and Detection: Critical for studying retroviruses (e.g., HIV, HTLV) and other RNA viruses by converting viral RNA into DNA for amplification and sequencing.
• Next-Generation Sequencing (NGS) Sample Prep: Used in the preparation of RNA-seq libraries by generating cDNA from RNA templates.
• Molecular Cloning: Facilitating the insertion of genes expressed from RNA into plasmid vectors for protein expression and functional studies.
• Diagnostic Kit Manufacturing: A key component in the production of commercial kits for infectious disease testing and genetic screening.

Basic Information

Product Name	Reverse Transcriptase
CAS No.	9068-38-6
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	Approx. 66-71 kDa (subunit) - Contact for details
Synonyms	RNA-directed DNA polymerase; Deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed); RTase; Avian Myeloblastosis Virus Reverse Transcriptase (AMV-RT); Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT); HIV-1 Reverse Transcriptase; RNA-dependent DNA polymerase; Transcriptase reverse
EINECS	Contact for details

Quality Control

Our Reverse Transcriptase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing for activity, purity, and absence of contaminating nucleases (RNase and DNase). Certificates of Analysis (COA) detailing specific activity, purity by SDS-PAGE, and functional performance in standard assays are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a buffered glycerol solution. Keep the product in its original container under the recommended conditions.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Converts RNA template to cDNA as per assay
Specific Activity	>200,000 units/mg protein
Purity (SDS-PAGE)	>95%
Contaminating RNase Activity	Not detectable
Contaminating DNase Activity	Not detectable
Functional Test (RT-PCR)	Amplifies target from control RNA
Storage Buffer	50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.