Description

Aminopeptidase M CAS NO 9054-63-1 is a high-purity, zinc-containing metalloprotease enzyme that catalyzes the hydrolysis of N-terminal amino acid residues from peptides and proteins. This specificity makes it an indispensable tool for protein sequencing, peptide mapping, and the production of bioactive peptides. It is essential for research and development laboratories in the pharmaceutical, biotechnology, and food science industries, as well as for manufacturers of diagnostic kits and specialty biochemicals.

Application

• Protein Sequencing & Peptide Mapping: Used to determine the N-terminal amino acid sequence of proteins and peptides, a critical step in structural characterization.
• Bioactive Peptide Production: Employed in the enzymatic synthesis and modification of peptides for pharmaceutical and nutraceutical applications.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assays and diagnostic kits for clinical and research use.
• Biotechnology Research: Utilized in proteomics research, enzyme kinetics studies, and the investigation of protein-protein interactions.
• Food Science & Flavor Development: Applied in the controlled hydrolysis of food proteins to generate flavor peptides or reduce bitterness.
• Amino Acid Analysis: Serves as a preparatory enzyme to liberate N-terminal amino acids for subsequent analytical identification.

Basic Information

Product Name	Aminopeptidase M
CAS No.	9054-63-1
Molecular Formula	N/A (Enzyme from porcine kidney microsomes)
Molecular Weight	~280,000 Da (approximate, multimeric glycoprotein)
Synonyms	Leucine Aminopeptidase M; LAP-M; Microsomal Aminopeptidase; Aminopeptidase N; CD13; Alanyl Aminopeptidase; Membrane Alanyl Aminopeptidase; EC 3.4.11.2; Glycoprotein 150; Kidney Aminopeptidase M
EINECS	Contact for details

Quality Control

Our Aminopeptidase M is produced and tested under a rigorous quality management system. Each lot is characterized for specific activity, purity, and functionality to ensure batch-to-batch consistency for your critical applications. A comprehensive Certificate of Analysis (COA) detailing parameters such as activity units, protein content, and zymogram purity is provided with every shipment. We adhere to relevant quality guidelines suitable for research and industrial use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Identification (Activity)	Conforms
Specific Activity	> 10 units/mg protein (Typical)
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE / Zymogram)	Major band at ~150 kDa
pH Optimum	6.5 - 7.5
Temperature Optimum	37°C
Inhibitors	Inhibited by EDTA, 1,10-Phenanthroline, Bestatin

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.