Description

Ribonuclease H CAS NO 9050-76-4 is a specific endonuclease enzyme that selectively degrades the RNA strand in RNA-DNA hybrid molecules. This precise catalytic activity makes it a critical reagent in molecular biology research and biotechnological applications. It is essential for laboratories and companies engaged in genetic analysis, reverse transcription studies, and nucleic acid manipulation.

Application

• Reverse Transcription Research: Used to remove RNA templates from cDNA synthesis reactions, improving the efficiency and purity of cDNA production.
• Molecular Cloning: Critical in protocols for the removal of mRNA during the preparation of cDNA libraries.
• Nuclease Protection Assays: Employed to specifically digest unhybridized RNA probes, allowing for accurate mapping and quantification of RNA molecules.
• Oligonucleotide Synthesis & Purification: Aids in the removal of RNA contaminants from synthetic DNA oligonucleotides.
• Virology Studies: Used in research on retroviruses (like HIV) to study the reverse transcription process where RNase H activity is essential.
• Diagnostic Kit Manufacturing: Incorporated as a key enzyme component in diagnostic kits for pathogen detection and genetic testing.

Basic Information

Product Name	Ribonuclease H
CAS No.	9050-76-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	RNase H; Ribonuclease H (E. coli); Endoribonuclease H; RNase H I; Ribonuclease HI; EC 3.1.26.4; RNA-DNA hybrid ribonuclease; Hybridase
EINECS	Contact for details

Quality Control

Our Ribonuclease H is produced and tested under stringent quality management systems. Each batch is subjected to rigorous analytical testing to ensure high specific activity, purity, and the absence of contaminating nucleases (e.g., DNase, RNase A). A comprehensive Certificate of Analysis (COA) detailing activity units, purity, and stability data is provided with every shipment to guarantee reproducible performance in your sensitive applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquotting upon receipt is recommended for frequent use to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (Activity)	Cleaves RNA in an RNA-DNA hybrid
Specific Activity	> 20,000 units/mg protein
Purity (SDS-PAGE)	> 95%
DNase Contamination	Not detectable
RNase A Contamination	Not detectable
Protein Concentration	Provided on COA
Storage Buffer	50 mM Tris-HCl, pH 7.5, 50% Glycerol (for liquid forms)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.