Description

Sephadex G-50 is a cross-linked dextran gel, widely recognized as a size-exclusion chromatography medium for the separation and purification of biomolecules. Its core value lies in its ability to efficiently fractionate peptides, oligonucleotides, and small proteins based on molecular size, ensuring high-resolution results. This product is essential for researchers and process developers in the biotechnology, pharmaceutical, and life science industries who require reliable gel filtration for downstream processing and analytical applications.

Application

• Size-Exclusion Chromatography (SEC/GFC): For the separation and purification of peptides, oligonucleotides, and globular proteins in the molecular weight range of 1,500 to 30,000 Da.
• Desalting and Buffer Exchange: Rapid removal of salts, dyes, or other small molecules from protein and nucleic acid samples.
• Sample Preparation for Analysis: Clean-up step prior to HPLC, mass spectrometry, or electrophoresis to remove interfering contaminants.
• Fractionation of Biomolecules: Isolation of specific molecular weight fractions from complex biological mixtures for further study.
• Pharmaceutical Downstream Processing: Purification steps in the manufacturing of therapeutic peptides and small proteins.
• Laboratory-Scale Process Development: Method optimization and scaling studies for bioprocess development.

Basic Information

Product Name	Sephadex G-50
CAS No.	9048-71-9
Molecular Formula	(C6H10O5)n
Molecular Weight	High polymer (Variable)
Synonyms	Dextran gel; Cross-linked dextran; Gel filtration medium G-50; Sephadex G50; Dextran cross-linked with epichlorohydrin; G-50 Fine; G-50 Medium; G-50 Superfine; Gel Filtration Chromatography Medium; Size Exclusion Gel
EINECS	Contact for details

Quality Control

Our Sephadex G-50 is manufactured under a quality management system to ensure batch-to-batch consistency for critical research and process applications. Each lot is tested for key parameters including particle size distribution, exclusion limits, swelling characteristics, and flow properties. Certificates of Analysis (COA) detailing these specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect swelling and performance.

Specification

Item	Specification
Appearance	White to off-white free-flowing powder
Particle Size (Fine Grade)	20 - 80 μm
Exclusion Limit (Globular Proteins)	~30,000 Da
Bed Volume (Swollen in water)	9 - 11 mL/g
pH Stability (Long Term)	2 - 13
Operating Temperature	4 - 40°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.