Description

Ec 1.1.3.21 CAS NO 9046-28-0 is a high-purity enzyme preparation, specifically identified as L-lactate dehydrogenase (LDH). This biocatalyst is essential for biochemical research and industrial processes involving the interconversion of lactate and pyruvate. It is a critical reagent for diagnostic applications, enzymatic assays, and biomanufacturing workflows. Industries requiring precise enzymatic activity, including pharmaceuticals, diagnostics, and life science research, rely on this product for its consistent performance and quality.

Application

• Clinical Diagnostics: Key component in vitro diagnostic (IVD) kits for measuring lactate dehydrogenase (LDH) levels in blood serum, a crucial marker for tissue damage, heart attack, liver disease, and certain cancers.
• Biochemical Research: Used as a standard enzyme in research laboratories for studying glycolysis, cellular metabolism, anaerobic respiration, and cytotoxicity assays.
• Enzymatic Assay Reagent: Employed in coupled enzyme assays for the quantitative determination of substrates like pyruvate or NADH, facilitating high-throughput screening and metabolic profiling.
• Food & Beverage Analysis: Applied in food quality control and fermentation process monitoring to measure lactate concentration.
• Pharmaceutical Manufacturing: Utilized in enzymatic synthesis and as a process control enzyme in the production of certain chiral intermediates.
• Cell Culture & Biotechnology: Serves as an indicator enzyme in cell culture media to monitor cell health, proliferation, and the onset of apoptosis.

Basic Information

Product Name	Ec 1.1.3.21 (L-Lactate Dehydrogenase)
CAS No.	9046-28-0
Molecular Formula	Protein (Enzyme)
Molecular Weight	~140,000 Da (Tetramer)
Synonyms	L-Lactate Dehydrogenase; LDH; Lactate Dehydrogenase A; L-LDH; 1.1.1.27 (older classification); L-Lactate:NAD+ Oxidoreductase; Heart LDH; Muscle LDH; Lactate Dehydrogenase from rabbit muscle; Lactic Dehydrogenase.
EINECS	Contact for details

Quality Control

Our L-lactate dehydrogenase is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. Certificates of Analysis (COA) detailing parameters such as specific activity (U/mg protein), protein content, and purity are provided with every shipment. We adhere to relevant guidelines for reagent-grade enzymes suitable for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. For the specific storage buffer and optimal handling conditions, please refer to the accompanying technical data sheet.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ 300 U/mg protein (at 37°C, pH 7.5)
Protein Content (Biuret)	≥ 90% of total
Identification (IR / Electrophoresis)	Conforms to standard
Solubility	Clear solution in buffer
Loss on Drying	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.