Description

Isocitrate Lyase From Bacillus Stearothermophilus is a purified enzyme preparation derived from a thermophilic bacterial source. This enzyme is critical for catalyzing the cleavage of isocitrate into glyoxylate and succinate in the glyoxylate cycle, a key metabolic pathway. It is an essential reagent for biochemical research, metabolic pathway analysis, and industrial biocatalysis processes requiring high-temperature stability.

Application

• Biochemical Research & Assay Development: Used as a key component in enzymatic assays to study the glyoxylate cycle, metabolic flux, and enzyme kinetics.
• Metabolic Pathway Analysis: Essential for in vitro reconstruction of metabolic pathways to understand plant and microbial metabolism, particularly in organisms utilizing the glyoxylate bypass.
• Diagnostic Kit Manufacturing: Serves as a critical reagent in the development of diagnostic kits for metabolic disorders and specific clinical chemistry applications.
• Industrial Biocatalysis: Employed in specialized biocatalytic processes where its thermostable nature from Bacillus stearothermophilus offers advantages for reactions at elevated temperatures.
• Enzyme Activity Studies: Used to investigate enzyme inhibitors and activators, supporting drug discovery efforts targeting bacterial persistence or plant metabolism.
• Educational & Teaching Laboratories: Provides a stable and reliable enzyme for university-level biochemistry and molecular biology laboratory courses.

Basic Information

Product Name	Isocitrate Lyase From Bacillus Stearothermophilus
CAS No.	9045-78-7
Molecular Formula	Protein (Enzyme)
Molecular Weight	Varies by subunit composition. Contact for details.
Synonyms	Isocitratase; Isocitrate Glyoxylate-Lyase; ICL; Three-DS-Isocitrate Glyoxylate-Lyase; EC 4.1.3.1; Glyoxylate Bypass Enzyme; Thermostable Isocitrate Lyase; Bacillus stearothermophilus ICL
EINECS	Contact for details.

Quality Control

Our enzyme preparations are produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and stability to ensure reproducible performance in sensitive applications. Certificates of Analysis (COA) detailing activity units/mg protein, purity by SDS-PAGE, and absence of contaminating enzyme activities are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquot into smaller volumes after thawing to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Catalyzes the specific cleavage of isocitrate
Specific Activity	> X units/mg protein (Grade dependent)
Purity (SDS-PAGE)	> 90% (Research Grade)
Protein Content (Biuret/Bradford)	As per COA
pH (in solution)	7.0 - 7.5
Stability	Stable for 12 months at -20°C when stored as directed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.