Description

3':5'-Cyclic-Nucleotide 5'-Nucleotidohydrolase is a crucial enzyme that catalyzes the hydrolysis of 3',5'-cyclic nucleotides, such as cAMP and cGMP, to their corresponding 5'-nucleoside monophosphates. This activity is fundamental to cellular signaling pathways, making it an essential research tool for studying phosphodiesterase activity, cyclic nucleotide metabolism, and intracellular second messenger systems. It is primarily utilized by biotechnology firms, pharmaceutical research and development laboratories, and academic institutions focused on biochemistry, molecular biology, and drug discovery.

Application

• Biochemical Research: Essential for in vitro studies of cyclic nucleotide signaling cascades and phosphodiesterase (PDE) enzyme kinetics.
• Pharmaceutical Development: Used in high-throughput screening (HTS) assays to identify and characterize novel PDE inhibitors for therapeutic applications (e.g., cardiovascular, neurological diseases).
• Diagnostic Kit Manufacturing: A key component in enzymatic assay kits designed to measure cyclic nucleotide levels in biological samples.
• Enzyme Characterization: Serves as a standard or reference enzyme for comparative studies of PDE isozyme specificity and function.
• Cell Signaling Studies: Employed to manipulate intracellular cAMP/cGMP levels in cell-based experiments to elucidate downstream effects.
• Academic Teaching: Used in advanced biochemistry and enzymology laboratory courses to demonstrate enzyme kinetics and regulation principles.

Basic Information

Product Name	3':5'-Cyclic-Nucleotide 5'-Nucleotidohydrolase
CAS No.	9040-59-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cyclic Nucleotide Phosphodiesterase; PDE; 3',5'-Cyclic-AMP Phosphodiesterase; cAMP Phosphodiesterase; Cyclic AMP Phosphodiesterase; Nucleotide Pyrophosphatase; EC 3.1.4.17
EINECS	Contact for details

Quality Control

Our 3':5'-Cyclic-Nucleotide 5'-Nucleotidohydrolase is produced and tested under stringent quality management systems. Each lot undergoes rigorous analysis to ensure high specific activity, purity, and functional consistency. Key parameters assessed include protein concentration, specific activity (units/mg protein), and absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and storage buffer composition is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Store away from strong acids, bases, and oxidizing agents.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Protein Content (Biuret)	>90%
Specific Activity	>100 units/mg protein
Purity (SDS-PAGE)	Single major band
pH (in storage buffer)	7.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.